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Anti-Plexin-C1 (extracellular)-FITC Antibody

Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Cat #: APR-091-F
Alternative Name Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1 (Accession O60486). Extracellular, N-terminus.
Accession (Uniprot) Number O60486
Gene ID 10154
Homology Mouse – identical. Rat – 14 out of 15 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-091), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of Plexin-C1 by direct flow cytometry in live intact mouse J774 macrophage cell line:
    Cell surface detection of Plexin-C1 by direct flow cytometry in live intact mouse J774 macrophage cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F), (2.5µg).
  • Cell surface detection of Plexin-C1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of Plexin-C1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F), (5µg).
References
  1. Perälä. et al.  (2012), Differentiation83, 77.
  2. Chen. et al. (2012), Oncogene32, 4941.
  3. Scott. et al. (2009), J Invest Dermatol129, 954.
  4. König. et al. (2014), Eur J Immunol.  44,  2648.
Scientific background

Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ∼210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.

It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A–D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins 1. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).

Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer 2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis 3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 15/11/2021

Anti-Plexin-C1 (extracellular) Antibody (#APR-091) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize PLXNC1 from mouse, rat and human samples.

Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize PLXNC1 from mouse, rat and human samples.

For research purposes only, not for human use
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