Anti-PMCA2 Antibody

Plasma membrane Ca2+ ATPase 2, ATP2B2
    Cat #: ACP-002
    Alternative Name Plasma membrane Ca2+ ATPase 2, ATP2B2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2 (Accession P11506). 2nd intracellular loop.
        Anti-PMCA2 Antibody
    Accession (Uniprot) Number P11506
    Gene ID 24215
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/15 amino acid residues identical.
    RRID AB_2340922.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-PMCA2 Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
        1,2. Anti-PMCA2 Antibody (#ACP-002), (1:400).
        3,4. Anti-PMCA2  Antibody, preincubated with the negative control antigen.
      • The plasma membrane calcium ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells1.

        The PMCA and the sodium/calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations2.

        Since it transports Ca2+ into the extracellular space, the PMCA is also an important regulator of the Ca2+ concentration in the extracellular space3.

        The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain4.

        The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, and so is suited to maintaining Ca2+ at its normally very low levels. The NCX is better suited to removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat PMCA2. Anti-PMCA2 Antibody (#ACP-002) can be used in western blot analysis and has been designed to recognize PMCA2 from mouse, rat and human samples.

    For research purposes only, not for human use