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Anti-Presenilin-1 Antibody

PSEN1, PS-1, Psnl1
Cat #: AIP-011
Alternative Name PSEN1, PS-1, Psnl1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1 (Accession P97887). 3rd cytoplasmic loop (at the Psen1 CTF subunit).
Accession (Uniprot) Number P97887
Gene ID 29192
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756652.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat pancreas membrane (lanes 1 and 4), rat hippocampus (lanes 2 and 5) and mouse pancreatic cancer cell line (MS1) (lanes 3 and 6):
    Western blot analysis of rat pancreas membrane (lanes 1 and 4), rat hippocampus (lanes 2 and 5) and mouse pancreatic cancer cell line (MS1) (lanes 3 and 6):
    1-3. Anti-Presenilin-1 Antibody (#AIP-011), (1:500).
    4-6. Anti-Presenilin-1 Antibody, preincubated with Presenilin-1 Blocking Peptide (#BLP-IP011).
Immunohistochemistry
  • Expression of Presenilin-1 in mouse brain
    Expression of Presenilin-1 in mouse brain
    Immunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-Presenilin-1 Antibody (#AIP-011), (1:600). A. In hippocampus, PS-1 staining (green) appears in apical dendrites of pyramidal neurons (arrows). B. In cerebellum, an intense staining for PS-1 (green) appears in dendritic trees of Purkinje cells (arrows). A moderate staining is detected in the granule layer and in the soma of Purkinje and molecular layer interneurons. In both panels DAPI is used as the counterstain.
References
  1. Somavarapu, A.K. and Kepp, K.P. (2016) Neurobiol. Dis. 89, 147.
  2. Chan, S.L. et al. (2000) J. Biol. Chem. 275, 18195.
  3. Stutzmann, G.E. et al. (2004) J. Neurosci. 24, 508.
Scientific background

Presenilin-1 (PSEN1) is a transmembrane protein encoded by the PS1 gene. The protein is comprised of 9 transmembrane domains. The N- and C-termini of the protein are cytosolic and lumenal respectively. PSEN1, together with three other proteins - nicastrin, presenilin enhancer 2 and anterior pharynx-defective 1 form a protein complex named γ-Secretase. PSEN1 serves as the catalytic subunit of the γ-secretase complex. This complex, along with α- and β-secretases cleaves the amyloid precursor protein (APP). APP is the precursor for β-Amyloid fibrils which are the pathological hallmark of Alzheimer's disease (AD) and mutations in the PSEN1 gene have been implicated in AD pathophysiology. Currently, it remains unclear whether PSEN1 mutations cause disease by a loss of function or a gain of toxic function mechanism1.

PS1 mutations causing an overexpression of mutant human PSEN1 also increase the expression of ryanodine receptor 3 in PC12 cells. In addition, PC12 and cortical neuron cells expressing mutant PSEN1 exhibit increased calcium responses to caffeine compared with cells expressing wildtype PSEN1. This enhanced release of calcium is associated with increased cell vulnerability to β-Amyloid and caffeine induced cellular death. It has been hypothesized that PSEN1 and RyR interact directly2.

PS1 mutations also enhance inositol triphosphate (IP3)-mediated Ca2+ release in non-excitable and excitable cells. IP3-evoked Ca2+ responses are more than threefold greater in PS1M146V knock-in mice relative to non-transgenic controls. These mutations specifically disrupt intracellular Ca2+ release rather than reduce cytosolic Ca2+ buffering or clearance3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Presenilin-1 Antibody (#AIP-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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