Anti-Presenilin-2 Antibody

Psen2, Ps2, Psnl2, AD3LP, AD5, E5-1 STM-2
  • New
Cat #: AIP-012
Alternative Name Psen2, Ps2, Psnl2, AD3LP, AD5, E5-1 STM-2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DSRPGPEDGENTAQWR, corresponding to amino acid residues 33-48 of rat Presenilin-2 (Accession O88777). Intracellular, N-terminus.
    • Anti-Presenilin-2 Antibody
    Accession (Uniprot) Number O88777
    Gene ID 81751
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-Presenilin-2 Antibody
      Western blot analysis of rat heart membrane:
      1. Anti-Presenilin-2 Antibody (#AIP-012), (1:400).
      2. Anti-Presenilin-2 Antibody, preincubated with the negative control antigen.
    • Anti-Presenilin-2 Antibody
      Western blot analysis of mouse liver membrane:
      1. Anti-Presenilin-2 Antibody (#AIP-012), (1:200).
      2. Anti-Presenilin-2 Antibody, preincubated with the negative control antigen.
    • Anti-Presenilin-2 Antibody
      Western blot analysis of human liver carcinoma HepG2 (lanes 1 and 3) and rat pheochromocytoma PC12 (lanes 2 and 4) cell lysates:
      1, 2. Anti-Presenilin-2 Antibody (#AIP-012), (1:200).
      3, 4. Anti-Presenilin-2 Antibody, preincubated with the negative control antigen.
    References
    1. Johnson, D.S. et al. (2017) Cold Spring Harb. Perspect. Med. 7, a024067.
    2. Fluhrer, R. et al. (2009) J. Biol. Chem. 284, 13975.
    3. Wolfe, M.S. (2013) Biochim. Biophys. Acta 1828, 2886.
    4. Sannerud, R. et al (2016) Cell 166, 193.
    5. De Strooper, B. et al. (2012) Cold Spring Harb. Perspect. Med. 2, a006304.
    Scientific background

    Presenilin 2 (PS2) is the catalytic subunit of a tetrameric complex containing presenilin-1 or 2, anterior pharynx defective 1 (APH1), nicastrin, and PEN-21.

    The presenilin complex is the founding member of a unique class of GXGD aspartyl proteases that catalyze the cleavage of the transmembrane domains of Type I membrane proteins including amyloid precursor protein (APP) and Notch1. Other members of this protease family include signal peptide peptidases (SPP) and a variety of archaeal homologs2.

    PS1 and PS2 proteins have nine helical TM domains arranged with the hydrophilic, flexible N terminus in the cytosol and the C terminus protruding into the lumen or extracellular space3.

    PS2 shares similarity with PS 1 and the crucial difference between them appears to be a “[DIE]xxxL/I/M” motif present only in PS2 which interacts in a phosphorylation dependent manner with AP-1 complexes and targets PS2 to the late endosome/lysosome complex. The absence of this sequence motif in PS1 allows for the wider subcellular location of PS1 in cell membranes4.

    Presenilin mutations have been shown to be correlated with occurrence of familial Alzheimer's disease5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 19/03/2020

    Anti-Presenilin-2 Antibody (#AIP-012) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN2 from rat, mouse, and human samples.

    For research purposes only, not for human use
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