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Anti-proBDNF Antibody

Brain-derived neurotrophic factor precursor

Cat #: ANT-006
Alternative Name Brain-derived neurotrophic factor precursor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
  • Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.
Accession (Uniprot) Number P23560
Gene ID 627
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 16/17 amino acid residues identical.
RRID AB_2039758.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody is specific for proBDNF, it does not crossreact with proNGF, proNT-3 or mature BDNF.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, ip, wb
May also work in: ic*, ifc*
Western blot
  • Rat hippocampal neurons (Danelon, V. et al. (2016) Mol. Cell. Neurosci. 75, 81.).
  • Expression of proBDNF in rat cerebellum
    Expression of proBDNF in rat cerebellum
    Immunohistochemical staining of rat cerebellum with Anti-proBDNF Antibody (#ANT-006). proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.
  • Multiplex staining of BDNF and proBDNF in rat hippocampus
    Multiplex staining of BDNF and proBDNF in rat hippocampus
    Immunohistochemical staining of rat hippocampal dentate gyrus perfusion-fixed frozen sections using Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:300) and Anti-proBDNF Antibody (#ANT-006), (1:200). A. BDNF staining (red) appears in interneuron outlines (arrows) in the hilus (H) region and in the granule layer (G). B. proBDNF staining (green) in the same section appears in interneuron outlines (arrows) in the hilus (H) region and in the granule layer (G). C. Merge of panel A and panel B shows extensive co-localization of the two proteins.
  1. Lee R. et al. (2001) Science 294,1945.
  2. Egan, M.F. et al. (2003) Cell 112, 257.
  3. Pang, P.T. et al. (2004) Science 306, 487.
  4. Michalski, B. et al. (2003) Mol. Brain Res. 111, 148.
Scientific background

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).

All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.

Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.

The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer’s disease.

Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-proBDNF Antibody
Expression of proBDNF in mouse prefrontal brain cortex.Western blot analysis of mouse prefrontal brain cortex lysates using Anti-proBDNF Antibody (#ANT-006). proBDNF expression shown in control mice and mice treated with metamphetamine (METH).Adapted from Ren, Q. et al. (2015) Transl. Psychiatry 5, e666. with permission of SPRINGER NATURE.
Last update: 08/01/2023

Anti-proBDNF Antibody (#ANT-006) is a highly specific antibody directed against the prodomain region of human proBDNF. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry applications. It has been designed to recognize proBDNF from human, mouse, and rat samples. The antibody does not cross react with mature BDNF, pro- and mature NGF or mature NT-3.

For research purposes only, not for human use



Western blot citations
  1. Mouse brain lysate (1:600).
    Nguyen, L. et al. (2016) Neuroreport 27, 1004.
  2. Mouse brain lysate.
    Dong, C. et al. (2019) Pharmacol. Biochem. Behav. 177, 61.
  3. Mouse brain lysate.
    Ren, Q. et al. (2015) Transl. Psychiatry 5, e666.
Immunoprecipitation citations
  1. Rat hippocampal neurons.
    Danelon, V. et al. (2016) Mol. Cell. Neurosci. 75, 81.
More product citations
  1. de la Cruz-Morcillo, M.A. et al. (2016) Oncotarget 7, 34480.
  2. Dieni, S. et al. (2012) J. Cell Biol. 196, 775.
  3. Shulga, A. et al. (2012) J. Neurosci. 32, 1757.
  4. Aboulkassim, T. et al. (2011) Mol. Pharmacol. 80, 498.
  5. Akil, H. et al. (2011) PLoS One. 6, e25097.
  6. Andersen, O.A. et al. (2010) J. Biol. Chem. 285, 12210.
  7. Unsain, N. et al. (2009) J. Neurochem 111, 428.
  8. Fauchais, A-L. et al. (2008) J. Immunol. 181, 3027.
  9. Silhol, M. et al. (2008) Rejuven. Res. 11, 1031.
  10. Unsain, N. et al. (2008) Neuroscience 154, 978.
  11. Schnydrig, S. et al. (2007) Neurosci. Lett. 429, 69.
  12. Silhol, M. et al. (2007) Neuroscience 146, 962.
  13. Tan, J. and Shepherd, R.K. (2006) Am. J. Pathol. 169, 528.


Scientific Background

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