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Home › Products › Neurotrophic Factors and Receptors › Neurotrophins › Antibodies

Certificate of Analysis

Anti-proBDNF-ATTO Fluor-488 Antibody

Brain-derived neurotrophic factor precursor

A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488

Cat #: ANT-006-AG
Alternative Name Brain-derived neurotrophic factor precursor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Prodomain of the BDNF protein.
Accession (Uniprot) Number P23560
Gene ID 627
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 16 /17 amino acid residues identical.
RRID AB_10557039.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-006), and immunohistochemistry (labeled antibody).
Applications: fc, if, ih
May also work in: ic*
Immunohistochemistry
  • Rat dorsal root ganglion frozen sections (1:50).
  • Expression of proBDNF in rat DRG
    Expression of proBDNF in rat DRG
    Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-proBDNF-ATTO-488 Antibody (#ANT-006-AG), (1:50), (green). Staining is present in DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Direct flow cytometry
  • Human isolated platelets (Azoulay, D. et al. (2014) Br. J. Haematol. 164, 451.).
Scientific background

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).

All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.

Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.

The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer’s disease.

Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
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Last update: 08/06/2022

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For research purposes only, not for human use

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    Control Product

    • A Blocking peptide for Anti-proBDNF Antibody (#ANT-006) and Guinea pig Anti-proBDNF Antibody (#ANT-006-GP). Intended to be used as a negative control.

      proBDNF Blocking Peptide

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    • ATTO Fluorescent Dyes

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