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4-6. Guinea pig Anti-proBDNF Antibody, preincubated with the control peptide antigen.
All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.
Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.
The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the pro domain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP) a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer’s disease.
Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against the prodomain region of human proBDNF. Guinea pig Anti-proBDNF Antibody (#AGP-032), raised in guinea pig, can be used in western and immunohistochemistry applications. In addition, the antibody is an excellent tool for easy immuno-colocalization studies. It has been designed to recognize proBDNF from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-proBDNF Antibody (#ANT-006) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
Antibodies for immuno-colocalization experiments
- Anti-proBDNF-ATTO-488 Antibody (#ANT-006-AG). A fluorescent labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.
- Anti-proBDNF Antibody (#ANT-006)
- Anti-TrkB (extracellular) Antibody (#ANT-019)
- Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007)
- Recombinant human Val66Met proBDNF (cleavage resistant) protein (#B-456)
- Recombinant mouse proBDNF protein (#B-240)
- Recombinant human proBDNF protein (#B-257)
- Neurotrophic Factor Explorer Kit (#EK-500)