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Anti-SLC6A7 (Proline Transporter) Antibody

Sodium-dependent proline transporter, Brain-specific L-proline transporter, PROT

Cat #: AGT-013
Alternative Name Sodium-dependent proline transporter, Brain-specific L-proline transporter, PROT
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KPLMVHMRKYGGIT, corresponding to amino acid residues 602-615 of rat SLC6A7 (Accession P28573). Intracellular, C-terminus.
Accession (Uniprot) Number P28573
Gene ID 117100
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756640.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Anti-SLC6A7 (Proline Transporter) Antibody (#AGT-013), (1:400).
    3,4. Anti-SLC6A7 (Proline Transporter) Antibody, preincubated with SLC6A7/Proline Transporter Blocking Peptide (#BLP-GT013).
References
  1. Jayanthi, L.D. et al. (2000) Br. J. Pharmacol. 129, 465.
  2. Renick, S.E. et al. (1999) J. Neurosci. 19, 21.
  3. Kowalczuk, S. et al. (2005) Biochem. J. 386, 417.
  4. Shafqat, S. et al. (1995) Mol. Pharmacol. 48, 219.
  5. Thomas, F. et al. (1999) Mol. Cell. Neurosci. 13, 25.
Scientific background

Proline transporter (PROT) belongs to a gene family of high affinity Na+- and Cl-dependent plasma membrane transporters. PROT is a glycosylated protein encoded by the Slc6a7 gene. Its structure contains 12 putative transmembrane domains and is enriched in synaptosomal membrane fraction5.

PROT transporter is responsible for the active transporter of L-proline through the plasma membrane by using the transmembrane electrochemical ion gradients. Proline is co-transported with Na+ and Cl ions with a stoichiometry of 1:2:11-3

Several studies have indicated that in the mammalian CNS, L-proline acts as a putative synaptic regulatory molecule. L-proline shows several properties that are common with characterized amino acid neurotransmitters4. Thus, PROT can be used as an important target for therapeutic and pathological alterations of synaptic function2.

PROT transporter is expressed in subpopulations of putative glutamatergic pathways in the mammalian brain. Thus, PROT has a physiological role in excitatory neurotransmission.

Distribution of human PROT protein can be found in the corpus striatum suggesting that PROT is axonally transported from its site of biosynthesis to the nerve terminals in the striatum4,5.

Several recent studies have suggested that enkephalins competitively inhibit mammalian PROT through a direct interaction with the transporter protein at or near the substrate binding site1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Proline transporter. Anti-SLC6A7 (Proline Transporter) Antibody (#AGT-013) can be used in western blot analysis. It has been designed to recognize PROT from human, rat and mouse samples.

For research purposes only, not for human use
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