Free shipping starts now, no minimum, no coupons required!

Anti-proNT-3 Antibody

pro-Neurotrophin 3, proNTF3
Cat #: ANT-012
Alternative Name pro-Neurotrophin 3, proNTF3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783). Pro-domain of the NT-3 protein.
Accession (Uniprot) Number P20783
Gene ID 4908
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_2040015.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
Immunohistochemistry
  • Expression of proNT-3 in rat brain
    Expression of proNT-3 in rat brain
    Immunohistochemical staining of rat Islands of Calleja (IOC) using Anti-proNT-3 Antibody (#ANT-012). A. proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (star). B. The distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. C. The distribution of neuronal NOS is most intense in the cell-poor region.
References
  1. Hempstead, B. (2002) Curr. Opin. Neurobiol. 12, 260.
  2. Blum, R. and Konnerth, A. (2005) Physiology 20, 70.
Scientific background

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).

All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins. These are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.

Until recently, the functional role of the neurotrophin prodomain was thought to assist in the correct folding of the mature protein and the sorting of the neurotrophin into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.

It remains to be established whether proNT-3 has different biological functions than the mature NT-3 neurotrophin.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human proNT-3. Anti-proNT-3 Antibody (#ANT-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize proNT-3 from rat, mouse and human samples. The antibody does not cross reacts with mature NT-3, pro- and mature NGF or pro- and mature BDNF.

For research purposes only, not for human use
Shipping and Ordering information