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Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody

Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor, PRIPR

Cat #: APR-068-F
Alternative Name Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor, PRIPR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR (Accession P43253). Extracellular, 3rd loop.
Accession (Uniprot) Number P43253
Gene ID 292661
Homology Mouse – identical; human – 11/13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-068), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of PTGIR by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of PTGIR by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody 
          (#APR-068-F), (2.5µg).
  • Cell surface detection of PTGIR by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of PTGIR by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody 
          (#APR-068-F), (5µg).
  • Cell surface detection of PTGIR by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:
    Cell surface detection of PTGIR by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody 
          (#APR-068-F), (5µg).
References
  1. Breyer, R.M. et al. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 661.
  2. Stitham, J. et al. (2011) Front. Pharmacol. 2, 24.
Scientific background

Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.

Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30% of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.

PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/08/2022

Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize PGI2 from mouse, rat and human samples.

Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody (#APR-068-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize PGI2 from mouse, rat and human samples.

For research purposes only, not for human use
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