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Anti-Prostaglandin F2-α Receptor/PTGFR Antibody

Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor

Cat #: APR-067
Alternative Name Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RSQQHRQGRSHHLE, corresponding to amino acid residues 233-246 of rat Prostaglandin F2-α receptor (Accession P43118). Intracellular, 3rd loop.
Accession (Uniprot) Number P43118
Gene ID 25652
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 13/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6) lysates:
    1-3. Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067), (1:200).
    4-6. Anti-Prostaglandin F2-α Receptor/PTGFR Antibody, preincubated with Prostaglandin F2-α Receptor/PTGFR Blocking Peptide (#BLP-PR067).
Immunohistochemistry
  • Expression of Prostaglandin F2-alpha receptor in mouse hippocampus
    Expression of Prostaglandin F2-alpha receptor in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067) (1:200), followed by goat anti-rabbit-AlexaFluor-488. PTGFR staining (green) in mouse hippocampal dentate gyrus is detected in cell bodies (arrows). Cell nuclei are stained with DAPI (blue). Mouse brain hippocampal dentate gyrus sections were obtained two hours following kainate acid intraventricular injection.
References
  1. Zhang, J. et al. (2010) Front. Pharmacol. 1, 116.
  2. Makino, S. et al. (2007) Semin. Reprod. Med. 25, 60.
  3. De Rensis, F. et al. (2012) Theriogenology 77, 1.
  4. Ohyama, K. (2017) Biol. Pharm. Bull. 40, 616.
  5. Scher, J.U. and Pillinger, M.H. (2009) J. Investig. Med. 57, 703.
  6. Camu, F. et al. (1992) Drugs 5, 42.
  7. Agas, D. et al. (2013) J. Cell. Physiol. 228, 25.
  8. Matsou, A. and Anastasopoulos, E. (2018) Expert Opin. Investig. Drugs 10, 777.
Scientific background

Prostaglandins (PGs) are key lipid mediators derived from arachidonic acid that are involved in numerous physiological and pathological processes including inflammation and cardiovascular homeostasis. Each PG acts on its specific and distinct cell surface G-protein coupled receptor (GPCR) or peroxisome proliferator-activated receptor (PPAR)1.

All prostaglandins are composed of a cyclopentanone nucleus with two side chains. Currently, three classes of prostaglandins are recognized, and these are categorized on the basis of the number of double bonds present within the prostaglandin molecule and on the fatty acid from which they are derived.

Prostaglandin F2α (PGF2α) and its receptor (FP) are required for female reproductive functions such as luteolysis and parturition2. PGF2α was shown to be an important regulator of corpora luteal (CL) function, uterine contractility, ovulation, and embryo attachment in female swine. High affinity PGF2α receptors are present in the CL at all stages of the estrous cycle3.

Apart from its pivotal role in parturition, PGF2α and its receptor are been implicated in blood pressure regulation4, atherosclerosis and other inflammation-related disorders5, cardiovascular diseases6 and bone development7. Prostaglandin F2α analogs were the first prostaglandin agonists introduced for glaucoma treatment, its mechanism of action includes reduction of intracellular pressure by increasing the uveoscleral outflow8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PTGFR from human, mouse, and rat samples.

For research purposes only, not for human use
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