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Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Antigen Incl. Antigen Incl.
This antibody is shipped with its antigen FREE of charge!
Type: Polyclonal
Source: Rabbit
Reactivity: h, m, r
Immunogen
Peptide (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375). 3rd extracellular loop.
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution 100 µl double distilled water (DDW).
Control antigen storage after reconstitution -20°C.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-063), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic, lci
Direct flow cytometry 
Human THP-1 monocytic leukemia and mouse J774 macrophage cells (2.5 µg).
Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).
Direct flow cytometry of live intact mouse J774 macrophage cells:
___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).
Lyophilized PowderControl Antigen Included
Last update: 03/02/2019
For research purposes only, not for human use