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Home › Products › G-Protein Coupled Receptors › Prostanoid Receptors › Antibodies

Certificate of Analysis

Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody
In wishlist

Prostaglandin E2 receptor EP1 subtype, Prostaglandin E receptor 1, PTGER1
  • New
    New

    This product has recently been released.

Cat #: APR-063-F
  • Lyophilized Powder
    Lyophilized Powder

    This product is freeze dried. All water molecules have been removed.

  • Antigen Incl.
    Antigen Incl.

    This antibody is shipped with its antigen FREE of charge!

  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375). 3rd extracellular loop.
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-063), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
    Direct flow cytometry
    Human THP-1 monocytic leukemia and mouse J774 macrophage cells (2.5 µg).
    Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).
    Direct flow cytometry of live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Prostanoid EP1 Receptor (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderControl Antigen Included
    Back to product page MSDS
    Last update: 03/02/2019
    For research purposes only, not for human use
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