Anti-Prostanoid EP1 Receptor (extracellular) Antibody

Prostaglandin E2 receptor EP1 subtype, PTGER1
    Cat #: APR-063
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375). 3rd extracellular loop.
    Accession (Uniprot) Number P35375
    Gene ID 19216
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, lci, wb
    May also work in: ih, ip
    Western blot
    Western blot analysis of rat colon lysate (lanes 1 and 4), mouse kidney  lysate (lanes 2 and 5) and human HT-29 colon adenocarcinoma cell lysate (lanes 3 and 6):
    1-3. Anti-Prostanoid EP1 Receptor (extracellular) Antibody (#APR-063), (1:200).
    4-6. Anti-Prostanoid EP1 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry analysis of live intact human THP-1 monocytic leukemia cell line:
    ___  Unstained cells + goat-anti-rabbit-AlexaFluor-488.
    ___  Cells + Anti-Prostanoid EP1 Receptor (extracellular) Antibody (#APR-063), (1:20) + goat-anti-rabbit- AlexaFluor-488.
    Live cell imaging / Immunocytochemistry
    Expression of Prostanoid EP1 Receptor in human U-87 MG cells
    Immunocytochemical staining of live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells with Anti-Prostanoid EP1 Receptor (extracellular) Antibody (#APR-063), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
    References
    1. Funk, D.C. et al. (1993) J. Biol. Chem. 268, 26767.
    2. Zare, B. et al. (2011) Sci. Pharm. 79, 793.
    3. Sun, B. et al. (2013) Mol. Cell. Biochem. 378, 195.
    4. O’Callaghan, G. et al. (2013) Int. J. Cancer 133, 825.
    5. Bergmann, C. et al. (2007) Cancer Res. 67, 8865.
    Scientific background

    Prostaglandin (PG) E2 is involved in a number of physiological and pathophysiological events in many tissues throughout the body. The biological effects of PGE2 are mediated through interaction with specific membrane-bound G-protein coupled prostanoid EP2 receptors. There are four subtypes of the EP receptor, termed EP1, EP2, EP3 and EP4, which have  been defined on the basis of their different pharmacological profiles and signal transduction pathways1.

    EP1 is characterized by the presence of a helical bundle consisting of seven hydrophobic transmembrane helices (TM1-TM7), which are connected through six alternating extracellular and intracellular loops. The N-terminus is located on the extracellular side of the membrane, whereas the C-terminus occupies the intracellular side2.

    EP1 receptors are thought to mediate contraction of smooth muscle in various tissues such as the gastrointestinal tract, respiratory tract, vas deferens, myometrium and the iris sphincter muscle and may also be important for absorption inhibition in the small intestine as well as enhancement of neuronal activity3.

    Human colon cancer cells express the EP1 receptor. Furthermore, EP1 receptor antagonists inhibit chemically induced breast cancer development in rats and reduce the number of skin tumors per mouse following UVB exposure4,5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 31/01/2019

    Anti-Prostanoid EP1 Receptor (extracellular) Antibody (#APR-063) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and live cell imaging applications. It has been designed to recognize Prostanoid EP1 Receptor from mouse, rat, and human samples.

    For research purposes only, not for human use