- Peptide (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375). 3rd extracellular loop.
- Western blot analysis of rat colon lysate (lanes 1 and 4), mouse kidney lysate (lanes 2 and 5) and human HT-29 colon adenocarcinoma cell lysate (lanes 3 and 6):1-3. Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody (#APR-063), (1:200).
4-6. Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP1/PTGER1 (extracellular) Blocking Peptide (#BLP-PR063).
- Cell surface detection of prostanoid EP1 receptor in live intact human THP-1 monocytic leukemia cell line:___ Unstained cells + goat-anti-rabbit-AlexaFluor-488.
___ Cells + Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody (#APR-063), (1:20) + goat-anti-rabbit- AlexaFluor-488.
- Expression of prostanoid EP1 receptor in human U-87 MG cellsCell surface detection of prostanoid EP1 receptor in live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells with Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody (#APR-063), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
Prostaglandin (PG) E2 is involved in a number of physiological and pathophysiological events in many tissues throughout the body. The biological effects of PGE2 are mediated through interaction with specific membrane-bound G-protein coupled prostanoid EP receptors. There are four subtypes of the EP receptor, termed EP1, EP2, EP3 and EP4, which have been defined on the basis of their different pharmacological profiles and signal transduction pathways1.
EP1 is characterized by the presence of a helical bundle consisting of seven hydrophobic transmembrane helices (TM1-TM7), which are connected through six alternating extracellular and intracellular loops. The N-terminus is located on the extracellular side of the membrane, whereas the C-terminus is located on the intracellular side2.
EP1 receptors are thought to mediate contraction of smooth muscle in various tissues such as the gastrointestinal tract, respiratory tract, vas deferens, myometrium and the iris sphincter muscle and may also be important for absorption inhibition in the small intestine as well as enhancement of neuronal activity3.
Human colon cancer cells express the EP1 receptor. Furthermore, EP1 receptor antagonists inhibit chemically induced breast cancer development in rats and reduce the number of skin tumors per mouse following UVB exposure4,5.
Species reactivity key:
Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody (#APR-063) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and live cell imaging applications. It has been designed to recognize EP1 receptor from mouse, rat, and human samples.
- Human Blood eosinophils.
Durchschein, F. et al. (2019) Dig. Dis. Sci. 64, 2806.
- Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular)-FITC Antibody (#APR-063-F). This FITC-conjugated antibody can be used to detect EP1 receptor in live cell flow cytometry.
- Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062)
- Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064)
- Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065)
- Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066)
- Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068)
- Anti-Thromboxane A2 Receptor Antibody (#APR-069)
- Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-FITC Antibody (#APR-064-F)
- Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067)