Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody

Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor

Cat #: APR-068
Alternative Name Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR (Accession P43253). Extracellular, 3rd loop.
Accession (Uniprot) Number P43253
Gene ID 292661
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 11/13 amino acid residues identical.
RRID AB_2756763.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse heart membranes (lanes 1 and 4), mouse lung lysate (lanes 2 and 5) and rat lung membranes (lanes 3 and 6):
    Western blot analysis of mouse heart membranes (lanes 1 and 4), mouse lung lysate (lanes 2 and 5) and rat lung membranes (lanes 3 and 6):
    1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:400).
    4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody, preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).
  • Western blot analysis of human Jurkat T-cell leukemia cell (lanes 1 and 4), human THP-1 monocytic leukemia cell (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell (lanes 3 and 6) lysates:
    Western blot analysis of human Jurkat T-cell leukemia cell (lanes 1 and 4), human THP-1 monocytic leukemia cell (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell (lanes 3 and 6) lysates:
    1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:200).
    4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody, preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).
Indirect flow cytometry
  • Cell surface detection of PTGIR in live intact human MEG-01 megakaryoblastic leukemia cell line:
    Cell surface detection of PTGIR in live intact human MEG-01 megakaryoblastic leukemia cell line:
    ___ Unstained cells + goat anti-rabbit-AlexaFluor-647 secondary antibody.
    ___ Cells + Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:20) + goat anti-rabbit-AlexaFluor-647 secondary antibody.
References
  1. Breyer, R.M. et al. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 661.
  2. Stitham, J. et al. (2011) Front. Pharmacol. 2, 24.
Scientific background

Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.

Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30% of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.

PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat the PGI2 receptor. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068) can be used in western blot analysis. The antibody recognizes an extracellular epitope, ideal for detecting the receptor in living cells. It has been designed to recognize PGI2 receptor from human, mouse and rat samples.

For research purposes only, not for human use
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