Name: Anti-QRFPR/GPR103 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-033

Cat #: AGR-033

Alternative Name Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HNLTREQFIHRYGLR, corresponding to amino acid residues 18-32 of rat QRFP receptor (Accession P83858). Extracellular, N-terminus.

Accession (Uniprot) Number P83858

Gene ID 310327

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical; human – 13/17 amino acid residues identical.

RRID AB_2756630.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3), mouse brain membranes (lanes 2 and 4) and human LNCaP cell lysate (lanes 3 and 6):
1-3. Anti-QRFPR/GPR103 (extracellular) Antibody (#AGR-033), (1:500).
4-6. Anti-QRFPR/GPR103 (extracellular) Antibody, preincubated with QRFPR/GPR103 (extracellular) Blocking Peptide (#BLP-GR033).

References

Chartrel, N. et al. (2016) Front. Neurosci. 10, 549.
Chen, A. et al. (2016) J. Neurosci. 36,1823.

Scientific background

QRFP is a 26-amino acid peptide (QRFP-26) that, widely distributed among vertebrates, is a member of the RF amide peptide family harboring the arginine-phenylalanine-amide (Arg-Phe-NH₂) sequence motif in their carboxyl terminal. It is the cognate ligand of the human orphan receptor GPR103, a G protein-coupled receptor. Like other members of the super family, QRFP receptor has an extracellular N-terminal tail and intracellular C-terminus.

Its QRFP N-terminus binds with high affinity to GPR103 and has the efficacy to inhibit cAMP synthesis. QRFP has been shown to affect locomotor activity, energy homeostasis, reproduction, bone formation, blood pressure, hormone regulation, nociception, and appetite stimulation.

QRFP expressing neurons have shown to be expressed in various hypothalamic nuclei including the ventromedial hypothalamic nucleus, the lateral hypothalamic area, retrochiasmatic area and the arcuate nucleus^1,2. The receptor is detected in the CNS and in peripheral tissues, notably in bone, heart, kidney, pancreas and testis.

Increasing data suggest the ligand/receptor system plays an important role in the regulation of food intake and glucose homeostasis¹.

Lyophilized Powder

Last update: 22/05/2022

Anti-QRFPR/GPR103 (extracellular) Antibody (#AGR-033) is a highly specific antibody directed against an epitope of the rat QRFP receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the receptor in living cells. It has been designed to recognize GPCR GPR103 from rat, mouse, and human samples.

Order Summary

Anti-QRFPR/GPR103 (extracellular) Antibody

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

Related Products

Need Help With This Product?