Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody

H2R, HH2R, Gastric receptor I, Gastric receptor 1
    Cat #: AHR-002
    Alternative Name H2R, HH2R, Gastric receptor I, Gastric receptor 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2 (Accession P25102). 2nd extracellular loop.
    • Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody
    Accession (Uniprot) Number P25102
    Gene ID 25461
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical.
    RRID AB_2039917.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Will not recognize human histamine H2 receptor.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, wb
    May also work in: ip*, lci*
    Western blot
    • Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody
      Western blot analysis of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
      1,2. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:200). 
      3,4. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody
      Expression of Histamine H2 receptor in rat stomach
      Immunohistochemical staining of paraffin embedded section of rat stomach using Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:100). H2R (brown staining) is expressed in parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
    Immunocytochemistry
    • Mouse primary microglia (Apolloni, S. et al. (2017) Front. Immunol. 8, 1689.).
    Indirect flow cytometry
    • Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody
      Cell surface detection of Histamine H2 receptor in live intact rat basophilic leukemia cell lines:
      ___ Unstained cells + goat-anti-rabbit-FITC.
      ___ Cells+ Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:20) + goat-anti-rabbit-FITC.
    • The control antigen is not suitable for this application.
    References
    1. Hill, S.J. et al. (1997) Pharmacol. Rev. 49, 253.
    2. Del Valle, J. and Gantz, I. (1997) Am. J. Physiol. 273, G987.
    3. Kobayashi, T. et al. (2000) J. Clin. Invest. 105, 1741.
    Scientific background

    Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2

    The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including the brain, gastrointestinal tract and heart.

    The most studied physiological role of the H2 receptor is its involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.

    Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody
    Expression of Histamine H2 Receptor in mouse primary microglia.Immunocytochemical staining of mouse primary microglia using Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002). H2R expression (green) partially co-localizes with CD11b (red).Adapted from Apolloni, S. et al. (2017) Front. Immunol. 8, 1689. with permission of Frontiers.
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat histamine H2 receptor. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002) can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize H2R from rat and mouse samples. It will not recognize human H2R.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Immunohistochemistry citations
    1. Mouse intestine sections.
      Gao, C. et al. (2015) mBio 6, e01358.
    Immunocytochemistry citations
    1. Mouse primary microglia.
      Apolloni, S. et al. (2017) Front. Immunol. 8, 1689.
    Shipping and Ordering information