- Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
- Rat DRG frozen section (1:60).
- Multiplex staining of TRPV1 and NaV1.8 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (green), (1:60) and Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR), (red), (1:60). A. TRPV1 staining. B. NaV1.8 staining. C. Merge of A and B demonstrates partial co-localization of TRPV1 and NaV1.8 channels. Nuclei stained using DAPI as the counterstain (blue).
- Rat PC12 cells (1:25).
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1
According to IUPHAR the TRP family comprises three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5
TRPV1 channel has many activators; among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.
Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9
Species reactivity key:
Multiplex staining of TRPV1 and mGluR5 in rat DRGImmunohistochemical staining of rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:60). A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C. Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).