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Anti-Rat TRPV1 (VR1) (extracellular) Antibody

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
Cat #: ACC-029
Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
Immunogen
  • Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
Accession (Uniprot) Number O35433
Gene ID 83810
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Specific to rat TRPV1.
RRID AB_2040258.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Recognizes TRPV1 from rat samples only.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of TRPV1 in rat DRG:
    Western blot analysis of TRPV1 in rat DRG:
    1. Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).
    2. Anti-Rat TRPV1 (VR1) (extracellular) Antibody, preincubated with Rat TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029).
Immunohistochemistry
  • Expression of TRPV1 in rat DRG
    Expression of TRPV1 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO Fluor-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Live cell imaging / Immunocytochemistry
  • F11, rat DRG cell line (1:1000) (Goswami, C. et al. (2010) J. Cell Sci. 123, 2045.).
References
  1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
  2. Clapham, D.E. (2003) Nature 426, 517.
  3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
  4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
  5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
  6. Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790.
  7. Tominaga, M. et al. (1998) Neuron. 21, 531.
  8. Agopyan, N. et al. (2004) Am. J. Physiol .Lung .Cell .Mol. Physiol. 286, L563.
  9. Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1

According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

TRPV1 channel has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7

TRPV1 is expressed predominantly in nociceptors and in sensory neurons.

Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-Rat TRPV1 (VR1) (extracellular) Antibody
Expression of TRPV1 in rat TGNs.Immunocytochemical staining of living rat trigeminal ganglia neurons (TGNs). Extracellular staining of cells using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029). Adapted from Meng, J. et al. (2016) Sci. Rep. 6, 21226. with permission of Nature Publishing Group.

Last update: 16/08/2020

Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPV1 from rat samples only.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Live cell imaging citations
  1. Rat trigeminal ganglion neurons.
    Meng, J. et al. (2016) Sci. Rep. 6, 21226.
More product citations
  1. Sanz-Salvador, L. et al. (2012) J. Biol. Chem. 287, 19462.
  2. Goswami, C. et al. (2010) J. Cell Sci. 123, 2045.
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