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Anti-RIC3 Antibody

Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3
Cat #: ANC-020
Alternative Name Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3 (Accession Q8BPM6). Intracellular, C-terminus.
Accession (Uniprot) Number Q8BPM6
Gene ID 320360
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
RRID AB_2756679.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-RIC3 Antibody (#ANC-020), (1:200).
    2. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).
  • Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):
    Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):
    1-3. Anti-RIC3 Antibody (#ANC-020), (1:200).
    4-6. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).
Immunohistochemistry
  • Expression of RIC-3 in mouse olfactory bulb and fornix
    Expression of RIC-3 in mouse olfactory bulb and fornix
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RIC3 Antibody (#ANC-020), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. A. RIC-3 staining in mouse olfactory bulb shows staining in the external plexiform layer (EPL) and in some glomeruli (G). Arrows point at glomeruli with RIC-3 positive core. B. RIC-3 staining in mouse fornix is detected in neuronal profiles (vertical arrows) and neuronal processes (horizontal arrow). Cell nuclei are stained with DAPI (blue).
References
  1. Ben-David, Y. and Treinin, M. (2017) Oncotarget 8, 5662.
  2. Ben-David, Y. et al. (2016) Mol. Brain 9, 47.
  3. Safdie, G. et al. (2016) Mol. Biol. Cell 27, 2994.
  4. Mulcahy, M.J. et al. (2015) PLoS One 10, e0134409.
Scientific background

RIC-3 (Resistance to inhibitors of cholinesterase 3) is a conserved endoplasmic reticulum-resident protein that is known to be a chaperone for the nicotinic acetylcholine receptors (nAChR) and for the serotonin (5-HT3) channels1.

RIC-3 affects the maturation of multiple nAChRs which has been shown to facilitate nAChR assembly and trafficking3,4.

Multiple transcripts of RIC-3 displaying differential expression are detected in humans. The full-length transcript includes two transmembrane domains followed by a coiled-coil domain, a nonconserved N-terminus and C-terminus domain, which includes a second coiled-coil domain. The short isoform lacks the coiled-coil domains and is very conserved in mammals1,2,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-RIC3 Antibody (#ANC-020) is a highly selective antibody directed against an epitope of mouse protein RIC-3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize RIC3 from human, mouse, and rat samples.

For research purposes only, not for human use
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