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Anti-RIM1 Antibody

Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1
Cat #: AIP-014
Alternative Name Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1 (Accession Q9JIR4). Intracellular.
Accession (Uniprot) Number Q9JIR4
Gene ID 84556
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.
RRID AB_2756653.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-RIM1 Antibody (#AIP-014), (1:200).
    3,4. Anti-RIM1 Antibody, preincubated with RIM1 Blocking Peptide (#BLP-IP014).
References
  1. Han, Y. et al. (2015) J. Neurophysiol. 113, 255.
  2. Su, S.C. et al. (2012) Neuron 75, 675.
  3. Weiss, N. et al. (2011) Pflugers Arch. 461, 447.
Scientific background

RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β; RIM2α, 2β, 2γ; and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca2+ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α1 subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels1-3.

RIM proteins were found to be required for normal Ca2+ triggering of exocytosis. This interaction with Ca2+ channel significantly slows channel inactivation resulting in increased Ca2+ influx during action potentials.

Deficiency of RIM1 decreases presynaptic Ca2+ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat RIM1. Anti-RIM1 Antibody (#AIP-014) can be used in western blot analysis. It has been designed to recognize RIM1 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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