Anti-Robo1 (extracellular) Antibody

Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • New
Cat #: ANR-181
Alternative Name Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DGVLVSTQDSRIKQLE, corresponding to amino acid residues 491 - 506 of human Robo1 (Accession Q9Y6N7). Extracellular, N-term.
    Accession (Uniprot) Number Q9Y6N7
    Gene ID 6091
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Robo1 (extracellular) Antibody
      Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
      1, 2. Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200).
      3, 4. Anti-Robo1 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Robo1 (extracellular) Antibody
      Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 4), human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
      1-3. Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200).
      4-6. Anti-Robo1 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Robo1 (extracellular) Antibody
      Cell surface detection of Robo1 live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Robo1 (extracellular) Antibody (#ANR-181), (2.5 µg) + goat-anti-rabbit-FITC.
    References
    1. Jiang, Z. et al. (2019) Ther. Adv. Med. Oncol. 11, 1.
    2. Blockus, H. and Chedotal, A. (2016) Development 143, 3037.
    3. Li, H.S. et al. (1999) Cell 96, 807.
    4. Carr, L. et al. (2017) PLoS One 12, e0172736.
    5. Chedotal, A. (2007) Adv. Exp. Med. Biol. 621, 65.
    6. Su, X. et al. (2016) Int. J. Immunopharmacol. 38, 70.
    Scientific background

    Roundabout (Robo) receptors are a conserved family of four single pass transmembrane proteins1. All members have multiple immunoglobulin-like domains in the extracellular N-terminal region, three extracellular fibronectin type II domains, and cytoplasmic conserved domains. Robos also undergo preoteolytic processing, important for their signaling process2. They serve as receptors for Slit proteins, where upon activation lead to pleiotropic effects including axonal guidance1, axonal repulsion3,4, and neural migration5.

    Slit/Robo signaling mainly prevents axons from migrating to inappropriate locations during the assembly of the nervous system6.

    Robos are mainly expressed in the nervous system, but are also detected in vascular endothelial and muscle cells7. In non-neuronal tissues, Slit/Robo proteins are key oncogenes and tumor suppressors in various types of cancers. Robo1 expression is elevated in breast cancer and colorectal cancer, and has an overall oncogenic role1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 18/06/2020

    Anti-Robo1 (extracellular) Antibody (#ANR-181) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize Robo1 from human, rat, and mouse samples.

    For research purposes only, not for human use
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