Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363
  • New
Cat #: ASR-011-F
Alternative Name Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1 (Accession P21453). Extracellular, N-terminus.
        Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    Accession (Uniprot) Number P21453
    Gene ID 1901
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-011), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
      • Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
        Cell surface detection of S1PR1 in live intact human Jurkat T-cell leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody (#ASR-011-F), (5 µg).
        Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
        Cell surface detection of S1PR1 in live intact mouse TK-1 T-cell lymphoma cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody (#ASR-011-F), (5 µg).
    References
      • Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.

        Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.

        Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 14/07/2019

    Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples.

    Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody (#ASR-011-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use