Anti-Semaphorin 4D (extracellular) Antibody

CD100, Semaphorin-J, SEMA4D
  • New
Cat #: ASR-064
Alternative Name CD100, Semaphorin-J, SEMA4D
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EKQHEVYWKVSEDK, corresponding to amino acid residues 80-93 of mouse Semaphorin-4D (Accession O09126). Extracellular, N-terminus.
    Accession (Uniprot) Number O09126
    Gene ID 20354
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, ip, lci
      • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
        1,2. Anti-Semaphorin-4D (extracellular) Antibody (#ASR-064), (1:200).
        3,4. Anti-Semaphorin-4D (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
        1,2. Anti-Semaphorin-4D (extracellular) Antibody (#ASR-064), (1:200).
        3,4. Anti-Semaphorin-4D (extracellular) Antibody, preincubated with the control peptide antigen.
      • Indirect flow cytometry of live intact human Jurkat T-cell leukemia cells:
        ___ Cells.
        ___ Cells + goat-anti-rabbit-FITC.
        ___ Cells + Anti-Semaphorin-4D (extracellular) Antibody (#ASR-064), (2.5 µg) + goat-anti-rabbit-FITC.
      • Okuno, T. et al. (2011) FEBS Lett. 585, 3829.
      • Esnault, S. et al. (2014) Clin. Immunol. 150, 90.
      • Lontos, K. et al. (2018) Front. Endocrinol. (Lausanne) 9, 322.
      • Semaphorins are a family of soluble and membrane associated proteins characterized by a conserved “sema” domain. They were originally described as axonal growth and guidance proteins required to guide neuronal axons to the right targets. Today semaphorins are known to possess pleiotropic and important functions in several physiological and pathological processes, including heart development, vascular growth, tumor progression, immune cell regulation, angiogenesis and vasculogenesis. To date, more than 20 types of semaphorin proteins have been identified and classified into eight subclasses1,2. The conserved “sema” domain of the protein includes arginine–glycine–aspartate sequences. The C-terminal region includes an IgG-like domain, a transmembrane domain, and a short cytoplasmic tail that contains one tyrosine phosphorylation site and multiple sites for serine–threonine phosphorylation1,2.

        Semaphorin 4D (Sema4D), also known as CD100, is a 150-kDa membrane‐associated‐type class IV semaphorin and was the first semaphorin shown to have immune regulatory activity. Other Sema4D functions include inactivation of platelets, stimulation of angiogenesis, and regulation of bone formation. Sema4D is expressed in many tissues including the brain, kidney, and heart. Sema4D is expressed in resting T cells, and expression is increased upon cellular activation3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 04/04/2019

    Anti-Semaphorin 4D (extracellular) Antibody (#ASR-064) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SEMA4D from rat, mouse, and human samples.

    For research purposes only, not for human use