Anti-Semaphorin 7A (extracellular) Antibody

CD108, JMH Blood Group Antigen, Semaphorin-K1, Semaphorin-L, SEMA7A
  • New
Cat #: ASR-077
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RKQEYNGKIPRFRR, corresponding to amino acid residues 190 - 203 of mouse Semaphorin-7A (Accession Q9QUR8). Extracellular.
    Accession (Uniprot) Number Q9QUR8
    Gene ID 20361
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, ip, lci
    Western blot
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1,2. Anti-Semaphorin 7A (extracellular) Antibody (#ASR-077), (1:200).
    3,4. Anti-Semaphorin 7A (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
    1. Anti-Semaphorin 7A (extracellular) Antibody (#ASR-077), (1:400).
    2. Anti-Semaphorin 7A (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry of live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Semaphorin 7A (extracellular) Antibody (#ASR-077), (5 µg) + goat-anti-rabbit-FITC.
    References
    1. Okuno, T. et al. (2011) FEBS Lett. 585, 3829.
    2. Esnault, S. et al. (2014) Clin. Immunol. 150, 90.
    Scientific background

    The semaphorins are a family of soluble and membrane associated proteins characterized by a conserved “sema” domain. They were originally described as axonal growth and guidance proteins required to guide neuronal axons to the right targets. Today, semaphorins are known to possess pleiotropic and important functions in several physiological and pathogenic processes, including heart development, vascular growth, tumor progression, immune cell regulation, angiogenesis and vasculogenesis1,2.

    To date more than 20 types of semaphorin proteins have been identified. Semaphorin-7A, also known as CD108, promotes olfactory bulb axon outgrowth in the nervous system. It is also required for the appropriate formation of the lateral olfactory tract during embryonic development1.

    Some of semaphorin-7A functions are mediated through β1-integrins and the transmembrane protein plexin C1 to which it binds exclusively. Studies show that in the airway remodeling of patients with chronic asthma, semaphorin 7A is a potentially important modulator of eosinophil profibrotic functions. In addition, semaphorin-7A plays an important role as an inducer of pulmonary fibrosis and liver fibrogenesis2.

    The semaphorin family has been classified into eight subclasses. Unlike other semaphorins, which are integral membrane proteins, semaphorin 7A is anchored to the membrane by a glycophosphatidylinositol (GPI)-linked sequence. The conserved amino‐terminal “Sema” domain of the protein includes arginine–glycine–aspartate sequence1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 23/10/2018

    Anti-Semaphorin 7A (extracellular) Antibody (#ASR-077) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize Semaphorin-7A from rat, mouse, and human samples.

    For research purposes only, not for human use