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Anti-Septin-7 Antibody

Sept7, CDC10, SEPT7A, NBLA02942, CDC3
Cat #: AIP-047
Alternative Name Sept7, CDC10, SEPT7A, NBLA02942, CDC3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HRIKKTVQVEQSK, corresponding to amino acid residues 83-96 of rat Septin-7 (Accession Q9WVC0). Intracellular.
Accession (Uniprot) Number Q9WVC0
Gene ID 64551
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat spleen (lanes 1 and 4), mouse spleen (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
    Western blot analysis of rat spleen (lanes 1 and 4), mouse spleen (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
    1-3. Anti-Septin-7 Antibody (#AIP-047), (1:200).
    4-6. Anti-Septin-7 Antibody, preincubated with Septin-7 Blocking Peptide (#BLP-IP047) (#BLP-IP047).
  • Western blot analysis of human Burkitt's lymphoma Daudi (lanes 1 and 3), human T-cell leukemia Jurkat (lanes 2 and 5) and human breast adenocarcinoma MCF-7 cell lysates (lanes 3 and 6):
    Western blot analysis of human Burkitt's lymphoma Daudi (lanes 1 and 3), human T-cell leukemia Jurkat (lanes 2 and 5) and human breast adenocarcinoma MCF-7 cell lysates (lanes 3 and 6):
    1-3. Anti-Septin-7 Antibody (#AIP-047), (1:200).
    4-6. Anti-Septin-7 Antibody, preincubated with Septin-7 Blocking Peptide (#BLP-IP047) (#BLP-IP047).
References
  1. Neubauer, K. and Zieger, B. (2017) Front. Cell Dev. Biol. 5, 3.
  2. Moore, K. et al. (2020) J. Cardiovasc. Dev. Dis. 7, 3.
  3. Valadares, N.P. et al. (2017) Biophys. Rev. 9, 481.
  4. Deb, B.K. et al. (2016) Nat. Commun. 7, 11751.
Scientific background

Septin 7 is a highly conserved GTP-binding filamentous protein. Septins are important for the organization of the actin cytoskeleton. Septin7 also plays an important role in cytokinesis, and membrane dynamics1,2.

Septins were first discovered in yeast and since, 13 septin genes have been identified in mammals (SEPT1 to SEPT12 and SEPT14). The 13 gene-encoded proteins can be classified into four distinct subgroups based on their sequence homology of their domain structure2.

Septins share a highly conserved structure composed of the central GTPase domain and N- and C-terminal domains of various lengths3. They self-assemble into higher-order structures, including filaments and rings in tri-, hexa-, or octameric complexes consisting of multiple septin polypeptides, which are typical for different cell types. The various septin isoforms seem to determine the function and regulation of the septin complex4.

Septin-7 regulates Ca2+ entry through Orai1 by regulating the opening of the channel5.

Dysregulation of septins leads to human diseases such as neurodegenerative and bleeding disorders. In non-dividing cells such as neuronal tissue and platelets septins have been associated with exocytosis1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 23/08/2020

Anti-Septin-7 Antibody (#AIP-047) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Septin-7 from human, rat, and mouse samples.

For research purposes only, not for human use
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