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Anti-SERCA2 Antibody

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2
Cat #: ACP-012
Alternative Name Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2 (Accession P11507). 2nd cytoplasmic loop.
Accession (Uniprot) Number P11507
Gene ID 29693
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2340924.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, wb
May also work in: ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat heart membranes (lanes 1 and 5), mouse heart membranes (lanes 2 and 6), rat PC12 pheochromocytoma cell line lysate (lanes 3 and 7) and human Jurkat T cell leukemia cell line lysate (lanes 4 and 8):
    Western blot analysis of rat heart membranes (lanes 1 and 5), mouse heart membranes (lanes 2 and 6), rat PC12 pheochromocytoma cell line lysate (lanes 3 and 7) and human Jurkat T cell leukemia cell line lysate (lanes 4 and 8):
    1-4. Anti-SERCA2 Antibody (#ACP-012), (1:200).
    5-8. Anti-SERCA2  Antibody, preincubated with SERCA2 Blocking Peptide (#BLP-CP012).
Immunocytochemistry
  • Expression of SERCA2 in mouse C2C12 cells
    Expression of SERCA2 in mouse C2C12 cells
    Immunocytochemical staining of fixed and permeabilized mouse myoblast C2C12 cells. A. Cells were stained using Anti-SERCA2 Antibody (#ACP-012), (1:300) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merged image of SERCA2 labeling with a live image of the cells. Cell nuclei were visualized using Hoechst 33342 (blue).
References
  1. Brini, M. and Carafoli, E. (2009) Physiol. Rev. 89, 1341.
  2. Vandecaetsbeek, I. et al. (2011) Cold Spring Harb. Perspect. Biol. 3, a004184.
  3. Clausen, J.D. et al. (2012) J. Biol. Chem. 287, 39460.
  4. Shi, B.J. et al. (2012) Int. J. Dermatol. 51, 1074.
Scientific background

Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.

In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.

SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat SERCA2. Anti-SERCA2 Antibody (#ACP-012) can be used in western blot and immunocytochemistry applications and was designed to recognize SERCA2 from rat, mouse and human samples.

For research purposes only, not for human use
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