Anti-SERCA3 Antibody

Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 3, Calcium pump 3, ATP2A3
  • New
Cat #: ACP-014
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)DEALKYLSRHHVD, corresponding to amino acid residues 981 - 993 of rat SERCA3 (Accession P18596). Cytoplasmic, C-terminus.
    Accession (Uniprot) Number P18596
    Gene ID 25391
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 11/13 amino acid residues identical; human – 10/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
    Western blot
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1,2. Anti-SERCA3 Antibody (#ACP-014), (1:200).
    3,4. Anti-SERCA3 Antibody, preincubated with the control peptide antigen.
    References
    1. Elaïb, Z. et al. (2016) Blood 128, 1129.
    2. Lipskaia, L. et al. (2014) Biochim. Biophys. Acta 1843, 2705.
    3. Erkasap, N. (2007) Anat. J. Cardiol. 1, 43.
    Scientific background

    SERCA3 belongs to the family of P-type ion translocating ATPases and is responsible for maintaining a Ca2+ concentration gradient between the cytosol and the endoplasmic reticulum. SERCA3 transports two Ca2+ ions in the endoplasmic reticulum (ER) per ATP molecule hydrolyzed1-3. SERCA proteins are transmembrane and consist of a single polypeptide chain folded into four major domains. SERCA proteins are encoded by 3 genes, ATP2A1, ATP2A2, and ATP2A3. All three genes produce several alternate transcripts and protein isoforms.

    SERCA3 protein is mostly expressed in non-muscle cells, especially in endothelial and hematopoietic cells. Minor expression can be detected in muscle cells2,3.

    SERCA3 knockout mice exhibit prolonged tail bleeding time and rebleeding1. Mutations in the SERCA3 gene are associated with type 2 diabetes. The protein is also implicated in several cardiac disorders2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/11/2018

    Anti-SERCA3 Antibody (#ACP-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SERCA3 from rat, mouse, and human samples.

    For research purposes only, not for human use