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Anti-SGLT2 (extracellular) Antibody

Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2
Cat #: AGT-032
Alternative Name Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)KDPAVGNISST(S)YQPR, corresponding to amino acid residues 242-257 of rat SGLT2 (Accession P53792). 3rd extracellular loop.
Accession (Uniprot) Number P53792
Gene ID 64522
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 15/16 amino acid residues identical; mouse - 14/16 amino acid residues identical; human - 12/16 amino acid residues identical.
RRID AB_2756649.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):
    Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):
    1,2. Anti-SGLT2 (extracellular) Antibody (#AGT-032), (1:200).
    3,4. Anti-SGLT2 (extracellular) Antibody, preincubated with SGLT2 (extracellular) Blocking Peptide (#BLP-GT032).
References
  1. Faham, S. et al. (2008) Science. 321, 810.
  2. Wright, E.M. et al. (2011) Physiol. Rev. 91, 733.
  3. Haas, B. et al. (2014) Nutr. Diabetes 4, e143.
Scientific background

The Na+/glucose symporter 2 (SGLT2) belongs to a family of solute sodium symporters (SSS). SSS proteins cotransport Na+ with sugars, amino acids, inorganic ions and vitamins. Members of this family are important in human physiology and disease.

SGLT2 is composed of a central group of seven helices (TM2, TM3, TM4, TM7, TM8, TM9, and TM11) that contribute side-chain interactions for ligand selectivity, along with seven supporting helices that stabilize these central helices. A striking feature is two discontinuous TM helices, TM2 and the symmetrically related TM7, in the center of the protomer1. The NH2 and COOH termini of the transporter are both extracellular.

Expression of SGLT2 mRNA occurs exclusively in the kidney and primarily in the first two segments of the proximal tubular system. SGLT2 is capable of reabsorbing about 90% of glucose from the primary urine produced in the kidneys.

SGLT2 symports a single Na+ molecule together with a glucose molecule. SGLT2 is selective for Na+ as the energizing cation. Apart from H+ and Li+, no other monovalent cation can replace Na+ to drive glucose transport2.

Renal SGLT2 inhibitors are new antidiabetic drugs with an insulin-independent mechanism of action. They pose one remarkable advantage compared with already established antidiabetics by increasing urinary glucose excretion without inducing hypoglycemia and thereby promoting body weight reduction2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-SGLT2 (extracellular) Antibody (#AGT-032) is a highly specific antibody directed against an epitope of the rat sodium/glucose cotransporter 2. The antibody can be used in western blot analysis. It has been designed to recognize SGLT2 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Porcine coronary artery sections (1:100).
    Khemais-Benkhiat, S. et al. (2020) J. Cell. Mol. Med. 24, 2109.
Immunofluorescence citations
  1. Porcine coronary artery sections (1:100).
    Khemais-Benkhiat, S. et al. (2020) J. Cell. Mol. Med. 24, 2109.
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