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Anti-Shank3 Antibody

SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, Proline-rich synapse-associated protein 2, ProSAP2
Cat #: APZ-013
Alternative Name SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, Proline-rich synapse-associated protein 2, ProSAP2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EKLPGSLRKGIPRTK, corresponding to amino acid residues 841-855 of rat Shank3 (Accession Q9JLU4). Intracellular, between the PDZ and the SAM domains.
Accession (Uniprot) Number Q9JLU4
Gene ID 59312
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2756772.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions:
    1,2. Anti-Shank3 Antibody (#APZ-013), (1:500).
    3,4. Anti-Shank3 Antibody, preincubated with Shank3 Blocking Peptide (#BLP-PZ013).
Immunohistochemistry
  • Expression of Shank3 in rat and mouse parietal cortex
    Expression of Shank3 in rat and mouse parietal cortex
    Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections using Anti-Shank3 Antibody (#APZ-013), (1:400), followed by anti-rabbit-Cy3 antibody (red). Shank3 staining of both rat (A) and mouse (B) brain sections, appears in neuron processes (horizontal arrows). Nuclei are stained with DAPI (blue).
References
  1. Grabrucker, A.M. et al. (2011) Trends Cell Biol. 21, 594.
  2. Jiang, Y. H. and Ehlers, M.D. (2013) Neuron 78, 8.
  3. Uchino, S. et al. (2013) Brain Dev. 35, 106.
  4. Lim, S. et al. (1999) J. Biol. Chem. 274, 29510.
  5. Wang, X. et al. (2014) Mol. Autism 5, 30.
  6. Gauthier, J. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 7863.
Scientific background

Shank3/ProSAP2 is one of three members of the Shank/ProSAP family of proteins which contain five conserved protein domains – an ankyrin repeat (ANK), a Src homology 3 (SH3), a PSD-95/Discs large/ZO-1 (PDZ), a proline-rich region containing homer- and cortactin-binding sites (Pro), and a sterile alpha motif (SAM)1. Shank proteins localize in the postsynaptic density (PSD) of excitatory synapses where they function as master scaffolding proteins by interacting directly or indirectly with various proteins, including major types of glutamate receptors – NMDARs, AMPARs, and mGluRs – via different domains2. Shank3 plays important roles in the formation, maturation, and maintenance of synapses3.

Shank3 is expressed abundantly in heart and moderately in brain (including the cortex, hippocampus and cerebellum) and spleen4.

Human genetic studies strongly support the notion that molecular defects of Shank3 contribute to autism spectrum disorders (ASD) which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests5. In addition, Shank3 mutations were also reported in patients with childhood-onset schizophrenia and intellectual disability6.

Furthermore, haplo insufficiency of Shank3 gene causes a developmental disorder, 22q13.3 deletion syndrome (known as Phelan–McDermid syndrome), that is characterized by severe expressive language and speech delay, hypotonia, global developmental delay, and autistic behavior3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Shank3. Anti-Shank3 Antibody (#APZ-013) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Shank3 from rat, mouse and human samples.

For research purposes only, not for human use
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