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Anti-SLC11A1/NRAMP1 (extracellular) Antibody

Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
Cat #: ANT-201
Alternative Name Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CANSSLHDYAKIFP(R), corresponding to amino acid residues 322-335 of human SLC11A1 (Accession P49279). 4th extracellular loop.
Accession (Uniprot) Number P49279
Gene ID 6556
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, rat – 14/15 amino acid residues identical; mouse – 13/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat spleen lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 4) and mouse lung membranes (lanes 3 and 6):
    Western blot analysis of rat spleen lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 4) and mouse lung membranes (lanes 3 and 6):
    1-3. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200).
    4-6. Anti-SLC11A1/NRAMP1 (extracellular) Antibody, preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).
  • Western blot analysis of human THP-1 monocytic leukemia cells:
    Western blot analysis of human THP-1 monocytic leukemia cells:
    1. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200)
    2. Anti-SLC11A1/NRAMP1 (extracellular) Antibody, preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).
Indirect flow cytometry
  • Cell surface detection of SLC11A1 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of SLC11A1 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Blackwell, J.M. et al. (2003) Immunol. Lett. 85, 197.
  2. Zwilling, B.S. et al. (1999) Infect. Immunol. 67, 1386.
  3. Goswami, T. et al. (2001) Biochem. J. 354, 511.
  4. Qi, H. et al. (2014) Spectrochim. Acta A Mol. Biomol. Spectrosc. 122, 82.
Scientific background

Solute  carrier  family  11  member  a1  (Slc11a1) also known as natural  resistance  associated  macrophage protein  1 (NRAMP1) is  a proton/divalent  cation  antiporter,  known for its  role  in  regulating antimicrobial activity of macrophages1. The structure of Slc11a1 includes a common and conserved hydrophobic core of 10 transmembrane domains (TM) and either one or two non-conserved, highly hydrophobic TM domains4.

Slc11a1 delivers divalent cations such as Fe2+, Zn2+, and Mn2+ from the cytosol to the late endosome/lysosome acidic compartments where the Fenton reaction can use ferrous iron to generate toxic hydroxyl radicals2. Thus, providing one mechanism by which Slc11a1 could influence antimicrobial activity in macrophages.

Transition of metal ions is essential and participates in numerous cellular functions such as regulation of transcription. In addition, hundreds of enzymes require metal ions as co-factors3.  

Studies show that polymorphism in SLC11A1 is correlated to the occurrence of autoimmune and infectious disease susceptibility, as well as innate and adaptive immune responses to mycobacterial products/antigens. Therefore, research on this ion transporter is of high importance1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 23/08/2020

Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize NRAMP1 from rat, mouse, and human samples.

For research purposes only, not for human use
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