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Anti-SLC17A9 Antibody

Vesicular nucleotide transporter, VNUT
Cat #: ANT-085
Alternative Name Vesicular nucleotide transporter, VNUT
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SQKVQESERAFTYS, corresponding to amino acid residues 155-168 of mouse VNUT (Accession Q8VCL5). Intracellular, 2nd loop.
Accession (Uniprot) Number Q8VCL5
Gene ID 228993
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – 13/14 amino acid residues identical; rat – 12/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1,2. Anti-SLC17A9 Antibody (#ANT-085), (1:200).
    3,4. Anti-SLC17A9 Antibody, preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-SLC17A9 Antibody (#ANT-085), (1:400).
    2. Anti-SLC17A9 Antibody, preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
Immunohistochemistry
  • Expression of VNUT (SLC17A9) in rat hypothalamus
    Expression of VNUT (SLC17A9) in rat hypothalamus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC17A9 Antibody (#ANT-085), (1:2000), followed by goat anti-rabbit-AlexaFluor-488. A. VNUT immunoreactivity (green) in the paraventricular nucleus (PVN) of the hypothalamus is detected in neurons (vertical arrows) and axonal processes (horizontal arrows). 3V = third ventricle. B. VNUT staining (green) in the supra-optic nucleus (SON) of the hypothalamus is observed in neurons (vertical arrows) and axonal processes (horizontal arrows). OT = Optic tract. Cell nuclei are stained with DAPI (blue).
References
  1. Cao, Q. et al. (2014) J. Biol. Chem. 289, 23189.
  2. Harada, Y. et al. (2018) J. Biol. Chem. 293, 3770.
  3. Miras-Portugal, M.T. et al. (2019) Front. Cell. Neurosci. 13, 224.
  4. Moriyama, Y. et al. (2017) Purinergic Signal. 13, 387.
  5. Sawada, K. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 5683.
Scientific background

The vesicular nucleotide transporter (VNUT), also known as SLC17A9, is a member of the solute carrier superfamily, which contains ~300 proteins. Members of the SLC17 family are expressed in intracellular membranes and insulated vesicles such as the lysosomes1.

SLC17A9 has 430 amino acids and predicted to have 12 transmembrane domains with both the carboxyl- and N-termini facing the same direction5. Although the human SLC17A9 ortholog displays sequence-specific modification of its N-terminus, SLC17A9 is highly conserved across species, which is believed to dictate its substrate preference5.

SLC17A9 is widely expressed in many tissues, especially in ATP containing vesicles4. In the brain, SLC17A9 is highly expressed in different regions such as the cortex, cerebellum, hippocampus, and olfactory bulbs, but also in astrocytes and microglia cells. SLC17A9 expression is also evident outside the central nervous system, such as in the gastrointestinal tract and the lungs4.

Unlike other SLC17 members, SLC17A9 is highly specific for phosphorylated nucleotides with particularly high affinity for ATP3. Silencing of SLC17A9 in PC12 cells revealed its fundamental role in ATP homeostasis as it dramatically reduced ATP concentrations in storage and secretory vesicles. In addition, SLC17A9 also plays a role in the immune system where it activates neutrophils by influencing ATP storage in secretory vesicles2.

Under specific circumstances, SLC17A9 is involved in different pathological conditions. For example, in the spinal cord, SLC17A9 expression is associated with a general incline to feel pain3. Moreover, SLC17A9 is also involved in the development of glaucoma in mice, possibly by regulating ATP secretion3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-SLC17A9 Antibody (#ANT-085) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VNUT from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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