Anti-SLC35G1 (extracellular) Antibody

Solute carrier family 35 member G1, Partner of STIM1, POST, Transmembrane protein 20, C10orf60, TMEM20
  • New
Cat #: ANT-196
Alternative Name Solute carrier family 35 member G1, Partner of STIM1, POST, Transmembrane protein 20, C10orf60, TMEM20
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)SDTSGMEESYSGHLK, corresponding to amino acid residues 209-223 of human SLC35G1 (Accession Q2M3R5). 3rd extracellular loop.
    • Anti-SLC35G1 (extracellular) Antibody
    Accession (Uniprot) Number Q2M3R5
    Gene ID 159371
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/15 amino acid residues identical; rat – 11/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
    Western blot
    • Anti-SLC35G1 (extracellular) Antibody
      Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):
      1, 2. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:200).
      3, 4. Anti-SLC35G1 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-SLC35G1 (extracellular) Antibody
      Western blot analysis of rat heart (lanes 5 and 7) and mouse heart (lanes 6 and 8) lysates:
      5, 6. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:400).
      7, 8. Anti-SLC35G1 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-SLC35G1 (extracellular) Antibody
      Western blot analysis of human chronic myelogenous leukemia MEG-O1 cell line (lanes 9 and 10) lysates:
      9. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:400).
      10. Anti-SLC35G1 (extracellular) Antibody, preincubated with the negative control antigen.
    References
    1. Krapivinsky, G. et al. (2011) Proc. Natl. Acad. Sci. U.S.A. 108, 19234.
    2. Srikanth, S. and Gwak, Y. (2012) J. Physiol. 590, 4169.
    3. Lacruz, R.S. and Feske, S. (2015) Ann. N.Y. Acad. Sci. 1356, 45.
    Scientific background

    SLC35G1 (Solute carrier family 35 member G1) is a 10 transmembrane-spanning protein that interacts with STIM1 and is involved in calcium level regulation in the ER, plasma membrane and mitochondria1.

    Stromal interaction molecule 1 (STIM1) translocates within the ER membrane upon calcium depletion and interacts with Orai1 on the plasma membrane. STIM1 gates Orai1, allowing calcium to enter the cytoplasm, where it repletes the ER store via Calcium-ATPase pumps.

    SLC35G1 is located on the plasma membrane and ER. Upon calcium depletion, SLC35G1 binds STIM1 and moves it within the ER to localize it near the cell membrane. hence its role in regulating cellular calcium levels1,2.

    Due to its involvement in the STIM1-Orai1 axis, SLC35G1 mutations may have a role in immunodeficiency, muscular hypotonia, Stormorken syndrome and York platelet syndrome and many other calcium flux related syndromes3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 20/03/2020

    Anti-SLC35G1 (extracellular) Antibody (#ANT-196) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC35G1 from human, rat, and mouse samples.

    For research purposes only, not for human use
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