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Anti-SLC38A1 Antibody

Sodium-coupled neutral amino acid transporter 1, Snat1, Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Ata1, Glnt, Sa2, Sat1
Cat #: ANT-184
Alternative Name Sodium-coupled neutral amino acid transporter 1, Snat1, Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Ata1, Glnt, Sa2, Sat1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SKFISDRESRRSLTNSH, corresponding to amino acid residues 41-57 of rat SLC38A1 (Accession Q9JM15). Cytoplasmic, N-terminus.
Accession (Uniprot) Number Q9JM15
Gene ID 170567
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3), rat placenta (lanes 2 and 4), (1:800), mouse brain membrane (lanes 5 and 6), (1:200), human U-87 MG (lanes 7 and 9), and human Jurkat (lanes 8 and 10), (1:500) cell lysates:
    Western blot analysis of rat brain lysate (lanes 1 and 3), rat placenta (lanes 2 and 4), (1:800), mouse brain membrane (lanes 5 and 6), (1:200), human U-87 MG (lanes 7 and 9), and human Jurkat (lanes 8 and 10), (1:500) cell lysates:
    1, 2, 5, 7, 8. Anti-SLC38A1 Antibody (#ANT-184).
    3, 4, 6, 9, 10. Anti-SLC38A1 Antibody, preincubated with SLC38A1 Blocking Peptide (#BLP-NT184).
References
  1. McGivan, J.D. and Bungard, C.I. (2007) Front. Biosci. 12, 874.
  2. Gonzalez-Gonzalez, I.M. et al. (2005) Neuroscience 130, 61.
  3. Yamada, D. et al. (2019) Commun. Biol. 2, 346.
  4. Pochini, L. et al. (2014) Front. Chem. 2, 61.
  5. Bröer, S. et al. (2014) Pflugers Arch. 466, 155.
Scientific background

SNAT1 (SLC38A1, Sodium-coupled neutral amino acid transporter 1) is a 486 amino acids system A type sodium symporter that catalyzes the electrogenic Na+-dependent uptake of glutamine, among other substrates. SNAT1 is thought to be responsible for glutamine uptake into neurons1.

SNAT1 belongs to the β-family of solute carriers that includes three SLC families, SLC32, SLC36, and SLC38. SNAT1 is a member of the SLC38 family that includes 11 members, SNAT1 to SNAT11.

SNAT1 and SNAT2 are expressed in neurons and in glial cells all over the central nervous system, with high expression in perivascular glial profiles and lower expression in astrocytes of the cerebral parenchyma. This supports a role in controlling the amino acid permeability at the blood-brain barrier2. SNAT1 also acts as a positive regulator of mTORC1 signaling pathway in neurons and plays an important pathological role in the mechanism underlying neuronal survival after ischemia3. It was also shown to be up-regulated in specific types of cancer4.

The structure of SNAT proteins is predicted to have a 5+5 inverted repeat fold typical of the bacterial LeuT, with intracellular N-terminus and extracellular and C-terminal tail5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-SLC38A1 Antibody (#ANT-184) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SNAT1 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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