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Anti-SLC38A9 Antibody

Sodium-Coupled Neutral Amino Acid Transporter 9, Solute Carrier Family 38 Member 9, Up-Regulated In Lung Cancer 11, URLC11

Cat #: ANT-189
Alternative Name Sodium-Coupled Neutral Amino Acid Transporter 9, Solute Carrier Family 38 Member 9, Up-Regulated In Lung Cancer 11, URLC11
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GSAYKLKSYTEGYGK, corresponding to amino acid residues 101 - 115 of mouse SLC38A9 (Accession Q8BGD6). Intracellular, N-term.
Accession (Uniprot) Number Q8BGD6
Gene ID 268706
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, Human – 14 out of 15 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
SLC38A9 Blocking Peptide (#BLP-NT189)
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-SLC38A9 Antibody (#ANT-189), (1:200).
    3-4. Anti-SLC38A9 Antibody, preincubated with SLC38A9 Blocking Peptide (#BLP-NT189).
  • Western blot analysis of human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
    Western blot analysis of human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
    1-3. Anti-SLC38A9 Antibody (#ANT-189), (1:200).
    4-6. Anti-SLC38A9 Antibody, preincubated with SLC38A9 Blocking Peptide (#BLP-NT189).
Immunohistochemistry
  • Expression of SLC38A9 in mouse cingulate cortex.
    Expression of SLC38A9 in mouse cingulate cortex.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-SLC38A9 Antibody (#ANT-189), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC38A9 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with SLC38A9 Blocking Peptide (#BLP-NT189), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Saxton, R.A. and D.M. Sabatini. et al. (2017) Cell. 169(2): p. 361-371.
  2. Zoncu, R., et al. (2011) Science. 334(6056): p. 678-83.
  3. Jung, J., H.M. Genau, and C. Behrends. et al. (2015) Mol Cell Biol. 35(14): p. 2479-94.
  4. Rebsamen, M., et al. (2015) Nature. 519(7544): p. 477-81.
  5. Wang, S., et al. (2015) Science. 347(6218): p. 188-94.
  6. Bar-Peled, L., et al. (2012) Cell. 150(6): p. 1196-208.
  7. Sancak, Y., et al. (2010) Cell. 141(2): p. 290-303.
  8. Petit, C.S., A. Roczniak-Ferguson, and S.M. et al. (2013) Cell Biol. 202(7): p. 1107-22.
  9. Tsun, Z.Y., et al. (2013) Mol Cell. 52(4): p. 495-505.
Scientific background

Sodium-coupled neutral amino acid transporter 9 (SLC38A9) is a lysosomal amino acid transporter, involved in the regulation of mechanistic target of rapamycin complex 1 (mTORC1) activity in response to amino acid levels within the lysosomal lumen1,2. A sodium-couple amino acid transporter at the lysosome, SLC38A9, senses arginine from within the lysosome to convey arginine sufficiency to mTORC1.

SLC38A9 forms a complex with other lysosomal resident proteins including the v-ATPase and a pentameric complex called Ragulator3-5. v-ATPase function is necessary for mTORC1 activation, but the molecular mechanism of this regulation is currently unclear2. Ragulator binds strongly to the Rag GTPases and was shown to function as a guanine exchange factor (GEF) for RagA/B6,7. Potentially SLC38A9 and the v-ATPase are able to regulate mTORC1 activity by modulating Ragulator’s GEF activity. RagA/B is not the only Rag GTPase that is regulated, but a Folliculin-FNIP2 complex is a GAP for RagC/D8,9.

In short, SLC38A9 is a functional component of the lysosomal amino acid sensing machinery involved in the control of mTORC1 activity, that underlies the regulation of the metabolic status and cellular responses to growth factors, energy, glucose and amino acid levels 4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/08/2022

Anti-SLC38A9 Antibody (#ANT-189) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC38A9 from rat, mouse and human samples.

For research purposes only, not for human use
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