Anti-SLC6A8 Antibody

Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1
    Cat #: AGT-008
    Alternative Name Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RLAVPPRETWTRQMD, corresponding to amino acid residues 46-60 of rat creatine transporter (Accession P28570). Extracellular, C-terminus.
    • Anti-SLC6A8 Antibody
    Accession (Uniprot) Number P28570
    Gene ID 50690
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    RRID AB_2756639.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-SLC6A8 Antibody
      Western blot analysis of mouse heart (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:
      1-3. Anti-SLC6A8 Antibody (#AGT-008), (1:200).
      4-6. Anti-SLC6A8 Antibody, preincubated with the negative control antigen.
    • Anti-SLC6A8 Antibody
      Western blot analysis of human HepG2 liver hepatocellular carcinoma:
      1. Anti-SLC6A8 Antibody (#AGT-008), (1:200).
      2. Anti-SLC6A8 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-SLC6A8 Antibody
      Expression of creatine transporter in rat hippocampus and cortex
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A8 Antibody (#AGT-008), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Creatine transporter staining (green) in the hippocampal CA1 region is detected in apical dendrites (arrows). B. Creatine transporter immunoreactivity (green) in cortex is observed in soma and apical dendrites of neurons (arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Uemura, T. et al. (2017) Biol. Pharm. Bull. 40, 49.
    2. Fezai, M. et al. (2016) Cell. Physiol. Biochem. 40, 1231.
    3. Dodd, J.R. and Christie, D.L. (2001) J. Biol. Chem. 276, 46983.
    Scientific background

    Creatine transporter (CRT, SLC6A8) is a member of the superfamily of Na+/Cl--coupled transporters for neurotransmitters and organic osmolytes. Creatine Transporter is responsible transports creatine to the blood-brain barrier and into neurons of the central nervous system (CNS). It plays an important role in the storage and utilization of cellular phosphate-bound energy mediated by creatine kinase. It is essential for maintaining brain creatine levels and central nervous system (CNS) functions1,2.

    Creatine transporter is expressed in various tissues including brain, retina, skeletal muscle, and heart1. The transporter structure contains 12 membrane-spanning domains, a large extracellular loop containing sites for N-linked glycosylation between the third and fourth transmembrane domains. The amino- and carboxyl-termini of the transporter are intracellular3.

    Creatine transporter is encoded by the SLC6A8 gene and loss-of-function mutations result in creatine deficiency resulting in cerebral creatine deficiency syndromes (CCDSs). These syndromes are the second-most common cause of chromosome X-linked mental retardation. Creatine concentration in the brain is greatly reduced in these patients and they show various neurodevelopmental disorders, including delays in speech and language development, seizures, mental retardation, autism and epilepsy1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Anti-SLC6A8 Antibody (#AGT-008) is a highly specific antibody directed against an epitope of the rat Creatine transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC6A8 from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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