Anti-Slitrk2 (extracellular) Antibody

SLIT and NTRK-like family member 2, SLITL1
    Cat #: ASR-082
    Alternative Name SLIT and NTRK-like family member 2, SLITL1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)KASRPPKMRNRPTPR, corresponding to amino acid residues 301-315 of mouse Slitrk2 (Accession Q810C0). Extracellular, N-terminus.
    • Anti-Slitrk2 (extracellular) Antibody
    Accession (Uniprot) Number Q810C0
    Gene ID 245450
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-Slitrk2 (extracellular) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
      1,2. Anti-Slitrk2 (extracellular) Antibody (#ASR-082), (1:200).
      3,4. Anti-Slitrk2 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Slitrk2 (extracellular) Antibody
      Western blot analysis of human U-87 MG glioblastoma cell line lysate:
      1. Anti-Slitrk2 (extracellular) Antibody (#ASR-082), (1:200).
      2. Anti-Slitrk2 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Slitrk2 (extracellular) Antibody
      Expression of Slitrk2 in rat striatum
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Slitrk2 (extracellular) Antibody (#ASR-082), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Slitrk2 immunoreactivity (green) appears in striosomal patches (S). Cell nuclei are stained with DAPI (blue).
    1. Yamagata, A. et al. (2015) Sci. Rep. 5, 9686.
    2. Kang, H. et al. (2016) Front. Mol. Neurosci. 9, 104.
    Scientific background

    Slitrk2 (SLIT and NTRK-like family member 2) is a neuronal transmembrane protein widely expressed in the nervous system and found to affect neurite outgrowth1,2. Slitrk2 structure contains tandem leucine-rich repeat (LRR) domains and a single transmembrane domain2.

    In mammals, the Slitrk family consists of at least six members: Slitrk1- Slitrk6. All Slitrk members apart from Slitrk3 can induce both excitatory and inhibitory synapses1.

    The Slitrk family is responsible for controlling excitatory and inhibitory synapse formation by forming trans-synaptic adhesions with LAR receptor protein tyrosine phosphatases (PTPs)1,2.

    Studies show that mutations in SLITRK gene family are linked to several neuropsychiatric disorders including schizophrenia, autism spectrum disorders, Tourette syndrome, and obsessive-compulsive disorder.

    A single amino acid substitution in Slitrk2 (V89M) impairs synapse formation activity in cultured neurons, but does not affect surface transport, expression, or synapse inducing activity1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 27/01/2020

    Anti-Slitrk2 (extracellular) Antibody (#ASR-082) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize Slitrk2 from mouse, human, and rat samples.

    For research purposes only, not for human use
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