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Anti-sloβ1 (KCNMB1) Antibody

Maxi potassium channel beta, MaxiKbeta, Ca2+-activated K+ channel subunit β1, BKCa subunit β1, BK beta1, Charybdotoxin receptor subunit beta-1, K(VCA)beta-1, Slowpoke-beta

Cat #: APC-036
Alternative Name Maxi potassium channel beta, MaxiKbeta, Ca2+-activated K+ channel subunit β1, BKCa subunit β1, BK beta1, Charybdotoxin receptor subunit beta-1, K(VCA)beta-1, Slowpoke-beta
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide KKLVMAQKRGETRALC, corresponding to amino acid residues 2-17 of rat sloβ1 (Accession P97678). Intracellular, N-terminal part.
Accession (Uniprot) Number P97678
Gene ID 29747
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human, dog, rabbit - identical.
RRID AB_2040095.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat colon lysate:
    Western blot analysis of rat colon lysate:
    1. Anti-sloβ1 (KCNMB1) Antibody (#APC-036), (1:400).
    2. Anti-sloβ1 (KCNMB1) Antibody, preincubated with sloβ1/KCNMB1 Blocking Peptide (#BLP-PC036).
  • Human detrusor smooth muscle (DSM) cells (1:100) (Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.).
Immunohistochemistry
  • Rat smooth muscle samples.
Immunocytochemistry
  • Human detrusor smooth muscle (DSM) cells (1:100) (Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.).
References
  1. Knaus, H-G. et al. (1994) J. Biol. Chem. 269, 17274.
  2. Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
  3. Brenner, R. et al. (2000) Nature 407, 870.
  4. Fernández- Fernández, J.M. et al. (2004) J. Clin. Invest. 113, 1032.
Scientific background

sloβ1 is a member of a family of regulatory β subunits that control the activity of the large conductance Ca2+-activated K+ channel KCa1.1. The family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region. The four members of the family have a distinct tissue distribution with sloβ1 expressed almost exclusively in smooth muscle.

Functionally, sloβ1 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology.

In the past few years there has been a lot of interest regarding the role of the sloβ1 subunit in the regulation of vascular tone and hypertension. Studies in sloβ1 knockout mice have shown that loss of this subunit results in systemic hypertension. Conversely, a recent study showed that a commonly found gain-of-function sloβ1 variant has a protective effect against human diastolic hypertension.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat sloβ1. Anti-sloβ1 (KCNMB1) Antibody (#APC-036) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize sloβ1 from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Human dermal fibroblasts (1:200).
    Kicinska, A. et al. (2016) Biochem. J. 473, 4457.
  2. Mouse aorta (1:5000).
    Kunduri, S.S. et al. (2014) Physiol. Rep. 1, e00037.
  3. Rat mesenteric arteries (1:400).
    Shi, L. et al. (2013) Mech. Ageing Dev. 134, 416.
  4. Mouse pulmonary artery smooth muscle cells (PASMCs).
    Zheng, Y.M. et al. (2013) Am. J. Physiol. 304, C780.
  5. Mouse cerebral artery smooth muscle cells (CASMCs).
    Zheng, Y.M. et al. (2013) Am. J. Physiol. 304, C780.
Immunohistochemistry citations
  1. Rat mesenteric arteries (1:200).
    Shi, L. et al. (2013) Mech. Ageing Dev. 134, 416.
Immunocytochemistry citations
  1. Rat pinealocytes.
    Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.
More product citations
  1. Rueda, A. et al. (2013) PLoS ONE 8, e53321.
  2. Shi, L. et al. (2013) J. Gerontol. A Biol. Sci. Med. Sci. doi:10.1093/gerona/glt205.
  3. Shi, L. et al. (2013) Exp. Physiol. 98, 326.
  4. Yamamura, H. et al. (2012) Am. J. Physiol. 302, 1257.
  5. Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.
  6. Piwonska, M. et al. (2008) Neuroscience 153, 446.
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