- Peptide (C)HYDEEAIRTNPK, corresponding to amino acid residues 134-145 of rat KCNMB3 (Accession A7VL23). Extracellular loop.
- Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat pancreas membranes (lanes 2 and 4):1,2. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:1000).
3,4. Anti-sloβ3 (KCNMB3) (extracellular) Antibody, preincubated with sloβ3/KCNMB3 (extracellular) Blocking Peptide (#BLP-PC068).
- Expression of KCNMB3 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:300), followed by goat anti-rabbit-Alexa-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in cortical neurons (arrows). B. Pre-incubation of the antibody with sloβ3 (KCNMB3) (extracellular) Blocking Peptide (BLP-PC068), suppressed staining. Cell nuclei are stained with DAPI (blue).
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- Lorenz, S. et al. (2007) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 144B, 10.
Large-conductance, voltage- and Ca2+-activated K+ channels, also known as the BK channels, are widely expressed channels that couple changes in submembrane Ca2+ concentration to the regulation of electrical excitability1. BK channels are formed from four α-subunits arising from the Slowpoke (Slo) gene product2. In addition, in smooth muscle and cochlea, an accessory β-subunit can regulate BK channel gating profoundly. At present, four β subunits have been cloned in mammals. β subunits alter the Ca2+ sensitivity and gating kinetics of BK channels, greatly contributing to BK channel diversity. On the other hand, they modify the BK channel pharmacological properties, changing toxin binding and acting as receptors for drugs3.
Regulatory β subunits share a putative membrane topology, with two transmembrane segments connected by a 120- residue extracellular loop and with NH2 and COOH terminals oriented toward the cytoplasm4. Each β subunit has a tissue-specific expression and modulates channel function uniquely which provides a major mechanism for diverse BK channel phenotypes in various tissues.
β3 is highly expressed in kidney, heart, and brain5. The β3 gene (KCNMB3) associates with Slo1 α subunits and regulate BK channel function. In humans, the β3 gene contains four N-terminal alternative exons that produce four functionally distinct β3 subunits, β3a-d. Three variants, β3a-c, exhibit kinetically distinct inactivation behaviors6. A mutation in the β3 gene is linked to idiopathic epilepsy7.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat sloβ3. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068) can be used in western blot analysis. It has been designed to recognize sloβ3 from rat and mouse samples.