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Anti-SMCT1 (SLC5A8) Antibody

Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT

Cat #: AGT-036
Alternative Name Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GGRKQNLDPRYILTK, corresponding to amino acid residues 544 - 558 of human SMCT1 (Accession Q8N695). Intracellular, C-terminus.
Accession (Uniprot) Number Q8N695
Gene ID SLC5A8
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 13 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6):
    Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6):
    1-3. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 4-6. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
  • Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4):
    Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4):
    1-2. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 3-4. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
Immunohistochemistry
  • Expression of SMCT1 in rat supraoptic nucleus.
    Expression of SMCT1 in rat supraoptic nucleus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SMCT1 immunoreactivity (green) was detected in neuronal profiles (arrows). B. Pre-incubation of the antibody with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Ganapathy et al (2005), Biochem Soc Trans, 33, 237.
  2. Gupta et al (2006), Life Sciences, 21, 2419.
  3. Vergara-Jaque et al (2017), J Chem Inf Model., 57, 3043.
Scientific background

Sodium-coupled monocarboxylate transporter 1, SLC5A8, is an electrogenic sodium (Na+) and chloride (Cl-)-dependent sodium-coupled solute transporter that belongs to the Na+/glucose co-transporter gene family (SLC5) 1.

It is expressed in the colon, ileum, kidney and thyroid gland and it is involved in the transport of a variety of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate) 1.

SLC5A8 was found to be silenced in colon cancer, gastric cancer and possibly other cancer types and hence it is a candidate tumor suppressor gene. This is probably mediated through its role in transforming butyrate, a metabolite that is related to these types of cancer 2.

The structure of SLC5A8 was predicted based on its homology to sodium-iodide symporter (NIS) and on the fact that it is a part of the Solut Sodium Symporter (SSS) protein family. The structure contains 13 α-helical TM segments, with the N-terminus facing the extracellular medium and the C-terminus facing the cytoplasm, and a conserved sodium binding site 3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 24/01/2021

Anti-SMCT1 (SLC5A8) Antibody (#AGT-036) is a highly specific antibody directed against an epitope of the human SLC5A8. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC5A8 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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