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Anti-Somatostatin Receptor Type 1 (extracellular) Antibody

SSTR1, SS1R, SRIF-2, SRIF2A

Cat #: ASR-001
Alternative Name SSTR1, SS1R, SRIF-2, SRIF2A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide CSRGPGSGAADGMEE, corresponding to amino acid residues 26-40 of rat SSTR1 (Accession P28646). Extracellular, N-terminus.
Accession (Uniprot) Number P28646
Gene ID 25033
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/15 amino acid residues identical.
RRID AB_2040206.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
Immunohistochemistry
  • Mouse brain sections (1:400) (Lukomska, A. et al. (2020) Brain Struct. Funct. 225, 387.).
  • Expression of SSTR1 in rat parietal cortex.
    Expression of SSTR1 in rat parietal cortex.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Somatostatin Receptor Type 1 (extracellular) Antibody (#ASR-001), (1:1000), followed by goat anti-rabbit-Alexa-488. A. SSTR1 immunoreactivity (green) appears in neuron outlines – mostly axonal and dendritic (arrows).  B. Pre-incubation of the antibody with Somatostatin Receptor Type 1 (extracellular) Blocking Peptide (#BLP-SR001), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Hofland, LJ. and Lamberts, S.W. (2001) Ann. Oncol. 12, S31.
  2. Fombonne, J. et al. (2003) Reprod. Biol. Endocrinol. 1, 19.
  3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31.
  4. Schulz, S. et al. (2002) Gynecol. Oncol. 84, 235.
Scientific background

Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues.1 In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain it acts as a neurotransmitter in both a stimulatory and inhibitory manner.1,2

Somatostatin mediates its action via six high affinity G-protein coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3, SSTR4 and SSTR5) which are encoded by five genes.1,2 Expression of the different receptors is developmentally regulated in a time and tissue-specific manner.2

Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids and breast tumors.3 Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in in vivo imaging of tumors that are positive to somatostatin receptors.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 30/01/2023

Anti-Somatostatin Receptor Type 1 (extracellular) Antibody (#ASR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SSTR1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Mouse brain sections (1:400).
    Lukomska, A. et al. (2020) Brain Struct. Funct. 225, 387.

Specifications

Scientific Background

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