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Anti-Somatostatin Receptor Type 3 Antibody

SSTR3, SS3R, Smstr3

Cat #: ASR-003
Alternative Name SSTR3, SS3R, Smstr3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide KVRSTTRRVRAPSC, corresponding to amino acid residues 233-246 of rat SSTR3 (Accession P30936). Intracellular, between transmembrane domains 5 and 6.
Accession (Uniprot) Number P30936
Gene ID 171044
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 11/14 amino acid residues identical.
RRID AB_2040210.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003), (1:200).    
    2. Anti-Somatostatin Receptor Type 3 Antibody, preincubated with Somatostatin Receptor Type 3 Blocking Peptide (#BLP-SR003).
Immunohistochemistry
  • Expression of SSTR3 in mouse cerebellum
    Expression of SSTR3 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum with Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003). A. SSTR3 appears in Purkinje cells (red, arrow points at an example). B. Staining of Purkinje nerve cells with mouse anti parvalbumin (a calcium binding protein, green). C. Confocal merge of SSTR3 and parvalbumin demonstrates the co-localization of these proteins in Purkinje cells (arrow points at a Purkinje cell pointed at in A and B).
References
  1. Patel, Y.C. (1999) Front. Neuroendocrinol. 20, 157.
  2. Weckbecker, G. et al. (2003) Nat. Rev. Drug Disc. 2, 999.
  3. Culler, M.D. et al. (2002) Ann. Endocrinol. (Paris) 63, 2S5.
  4. Corleto, V.D. et al. (2004) Dig. Liver Dis. 36, S8.
Scientific background

Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as practically every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28 that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. SST induces in target cells a variety of physiological functions that include nueromodulation, cell secretion, cell proliferation and smooth muscle contractility.

SST acts on its multiple cell targets via a family of six receptors that are originated form five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3, SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell response via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.

SSTRs are expressed widely in both the brain and peripheral tissues but with receptor subtype variations between the different cell types. Thus, SSTR3 expression in the brain is primarily concentrated in the cerebellum with lesser expression in peripheral organs such as the spleen, the liver and the pituitary.

Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Alomone Labs is pleased to offer a highly specific antibody directed against a highly conserved epitope in the third intracellular loop of the rat SSTR3. Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003) can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize SSTR3 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Mouse brain sections (1:300).
    Lukomska, A. et al. (2020) Brain Struct. Funct. 225, 387.

Specifications

Scientific Background

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