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Anti-SORL1 (extracellular) Antibody

Sortilin Related Receptor 1, Low-Density Lipoprotein Receptor relative with 11 Ligand-Binding Repeats, LR11, Sorting Protein-Related Receptor Containing LDLR Class A Repeats, SORLA
Cat #: ANR-132
Alternative Name Sortilin Related Receptor 1, Low-Density Lipoprotein Receptor relative with 11 Ligand-Binding Repeats, LR11, Sorting Protein-Related Receptor Containing LDLR Class A Repeats, SORLA
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KRRTPHAT(S)FNGED, corresponding to amino acid residues 652 - 665 of human SORL1 (Accession Q92673). Extracellular, N-terminus.
Accession (Uniprot) Number Q92673
Gene ID 6653
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse, rat – 13/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200).
    3, 4. Anti-SORL1 (extracellular) Antibody, preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 6), human Colo-205 colon adenocarcinoma cell line lysate (lanes 3 and 7) and HepG2 hepatocellular carcinoma (lanes 4 and 8):
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 6), human Colo-205 colon adenocarcinoma cell line lysate (lanes 3 and 7) and HepG2 hepatocellular carcinoma (lanes 4 and 8):
    1-4. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200).
    5-8. Anti-SORL1 (extracellular) Antibody, preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).
Indirect flow cytometry
  • Cell surface detection of SORL1 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of SORL1 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-SORL1 (extracellular) Antibody (#ANR-132), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Barthelson, K. et al. (2020) J. Alzheimers Dis. Rep. 4, 123.
  2. Motoi, Y. et al. (1999) Brain Res. 833, 209.
  3. Jacobsen, L. et al. (1996) J. Biol. Chem. 271, 31379.
Scientific background

Sortilin-related receptor1 (SORL1, also known as LR11 or SORLA) is a receptor involved in intracellular sorting and trafficking of proteins into their respective subcellular compartments1.

Direction of proteins into their proper compartments is essential for normal cell function and defects in these pathways are thought to be important factors in the pathogenesis of Alzheimer’s disease (AD).

The full-length transcript of SORL1 encodes a 250 kDa, membrane-bound protein comprised of multiple functional domains. These include a vacuolar protein sorting 10 (VPS10) domain, five low-density lipoprotein receptor (LDLR) class B repeats, an epidermal growth factor-like (EGF-like) domain, eleven LDLR class A repeats, six fibronectin-type (FN-type) repeats, a transmembrane domain and a cytosolic domain containing recognition motifs for cytosolic adaptors1.

SORL1 is widely expressed in the human brain, particularly in neurons of the hippocampus and some nuclei of the brainstem and Purkinje cells, and has slightly weaker expression in neurons of the thalamus and the hypothalamus2. It is also expressed in other tissue types such as the testes, ovaries, thyroid, and lymph nodes3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 04/10/2020

Anti-SORL1 (extracellular) Antibody (#ANR-132) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SORL1 from human, rat, and mouse samples.

For research purposes only, not for human use
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