Anti-Sortilin (extracellular)-FITC Antibody

Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
  • New
Cat #: ANT-009-F
Alternative Name Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin (Accession Q99523). Extracellular domain.
    Accession (Uniprot) Number Q99523
    Gene ID 6272
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, Rat – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-009), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
      • Direct flow cytometry of live intact mouse J774 macrophage cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-Sortilin (extracellular)-FITC Antibody (#ANT-009-F), (2.5 µg).
        Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-Sortilin (extracellular)-FITC Antibody (#ANT-009-F), (2.5 µg).
      • Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has been lately identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, Sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.

        Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in brain, spinal cord, skeletal muscle and adipocytes.

        Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90% of sortilin is retained in intracellular compartments with only about 10% reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 04/04/2019

    Anti-Sortilin (extracellular) Antibody (#ANT-009) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples.

    Anti-Sortilin (extracellular)FITC Antibody (#ANT-009-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use