Overview
- Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2 (Accession P52592). 2nd intracellular loop.
- Rat heart and kidney (1:200). Human brain neuroblastoma (SH-SY5Y) and MS-1 cell lysate. It is recommended to add 0.5% Tween-20 to blocking and antibody solutions.
- Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012).
Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.
Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.
Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.