Name: Anti-S1PR2 (EDG5) Antibody
Brand: Alomone Labs
SKU: ASR-012

Overview

Cat #: ASR-012

Alternative Name Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2 (Accession P52592). 2^nd intracellular loop.

Accession (Uniprot) Number P52592

Gene ID 14739

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical.

RRID AB_10556618.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.95 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:
1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012).

Immunohistochemistry

Expression of Sphingosine 1-phosphate receptor 2 in rat lung
Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:100). S1PR2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.

References

Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
Hla, T. et al. (2001) Science 294, 1875.
Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
Rosen, H. et al (2009) Annu. Rev. Biochem. 78, 743.
Rosen, H and Liao, J. (2003) Curr. Opin. Chem. Biol. 7, 461.

Scientific background

Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin¹. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration².

Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)³.

Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle⁴. Unlike S1PR1, knockout of SIPR2 gene is not lethal⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse sphingosine 1-phosphate receptor 2. Anti-S1PR2 (EDG5) Antibody (#ASR-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize S1PR2 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 02/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

S1PR2/EDG5 Blocking Peptide (#BLP-SR012) is the original antigen used for immunization during Anti-S1PR2 (EDG5) Antibody (#ASR-012) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR2/EDG5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

S1PR2/EDG5 Blocking Peptide (#BLP-SR012)

Shipping and Ordering Information

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Anti-S1PR2 (EDG5) Antibody