Anti-S1PR3 (EDG3) (extracellular)-ATTO-488 Antibody

Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
    Cat #: ASR-013-AG
    Alternative Name Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3 (Accession Q9Z0U9). Extracellular, N-terminus.
    • Anti-S1PR3 (EDG3) (extracellular)-ATTO-488 Antibody
    Accession (Uniprot) Number Q9Z0U9
    Gene ID 13610
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; human - 9/12 amino acid residues identical.
    RRID AB_10919249.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-013), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, ih*, lci*
    Direct flow cytometry
    • Anti-S1PR3 (EDG3) (extracellular)-ATTO-488 Antibody
      Cell surface detection of S1PR3 in human Jurkat intact live cells:
      ___ Unstained cells.
      ___ Cells + Anti-S1PR3 (EDG3) (extracellular)-ATTO-488 Antibody (#ASR-013-AG), (5-10 µg/5x105 cells).
    1. Hla, T. and Brinkmann, V. (2011) Neurology 76, S3.
    2. Rosen, H. et al. (2007) Trends Immunol. 28, 102.
    3. Rosen, H. et al. (2009) Annu. Rev. Biochem. 78, 743.
    4. Jiang, L.I. et al. (2007) J. Biol. Chem. 282, 10576.
    5. Sanchez, T. and Hla, T. (2004) J. Cell. Biochem. 92, 913.
    Scientific background

    Sphingosine 1-phosphate (S1P) is an active byproduct of sphingomyelin metabolism. All cells can synthesize this biomolecule but the majority of its synthesis comes from erythrocytes and endothelial cells. In cases of inflammation, mast cells and platelets are the main source of sphingosine 1-phosphate1. S1P cellular functions could be intracellular, where it is synthesized. In addition, upon secretion, it circulates in the blood via its binding to high-density lipoproteins and albumin. When S1P reaches its target cell it could activate 5 different high affinity receptors belonging to the G-protein coupled receptor superfamily: Sphingosine 1-phosphate receptors, termed S1PR1-51.

    Stimulation of S1P receptors triggers a cascade of signaling events depending on the receptor and on the G-protein it couples. S1PR1 couples to Gi2. S1PR2 can couple to Gs, Gq or G12/13 and S1PR3-5 can couple to Gior G12/133-4. The pathways activated vary from Ca2+ mobilization, activation or inhibition of adenylate cyclase phospholipase C activation and more. Through the different signaling pathways these receptors activate, S1P1 receptors are implicated in adherens junction assembly, cytoskeletal changes, cell migration, proliferation and apoptosis5.

    S1PR3 is specifically detected in the brain, heart, spleen, liver, lung, thymus, kidney, testis and skeletal muscle3. S1PR1 and S1PR2 are generally expressed in the CNS, cardiovascular and immune systems1. S1PR4 is specifically expressed in the lymphoid tissue and S1PR5 in natural killer cells and olygodendrocytes1.

    Malfunction of S1P receptor signaling is reported in various disorders, for example multiple sclerosis and may be targets for the development of therapeutic drugs1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 23/03/2020

    Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013) is a highly specific antibody directed against an extracellular epitope of mouse sphingosine 1-phosphate receptor 3. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize S1PR3 from human, rat and mouse samples.

    Anti-S1PR3 (EDG3) (extracellular)-ATTO-488 Antibody (#ASR-013-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-S1PR3 (EDG3) (extracellular)-ATTO-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use



    Scientific Background


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