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Anti-SVCT2/SLC23A2 (extracellular) Antibody

Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2

Cat #: AST-022
Alternative Name Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EITVANGTAELLEH, corresponding to amino acid residues 191-204 of mouse SVCT2 (Accession Q9EPR4). 2nd extracellular loop.
Accession (Uniprot) Number Q9EPR4
Gene ID 54338
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 11/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).
    3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody, preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
  • Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 3) and human COLO 205 colon adenocarcinoma (lanes 2 and 4) cell line lysates:
    Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 3) and human COLO 205 colon adenocarcinoma (lanes 2 and 4) cell line lysates:
    1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).
    3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody, preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
Immunohistochemistry
  • Expression of SVCT2 in mouse hippocampus
    Expression of SVCT2 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of SVCT2 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of SVCT2 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Bürzle, M. et al. (2013) Mol. Aspects Med. 34, 436.
  2. Yu, X. et al. (2017) Cell Res. 27, 1020.
  3. May, J.M. (2012) Subcell. Biochem. 56, 85.
Scientific background

Vitamin C, or ascorbic acid, is an important antioxidant. It also participates in numerous cellular functions such as peptide amidation, myelin formation, synaptic potentiation, and protection against glutamate toxicity. Although it circulates in the plasma in micromolar concentrations, it reaches millimolar concentrations in most tissues. These high concentrations are thought to be generated and maintained by SVCT2, a Na+-dependent transporter1.

SVCT2 belongs to the SLC23 family of nucleobase-ascorbate transporters (NAT), that consists of SLCT1, SLCT2 and SLCT3 transporters2. The NAT family shares a structure that contains fourteen transmembrane segments that are organized into a pair of “7 + 7” inverted repeats. The intertwined repeats constitute two distinctive domains, namely the core domain and the gate domain2.

Neurons in the central nervous system (CNS) contain some of the highest ascorbic acid concentrations in mammalian tissues, and therefore targeted deletion of SVCT2 in mice causes widespread cerebral hemorrhage and death on post-natal day one. SCVT2 is also related to neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s. The expression of SVCT2 is relatively widespread, where it serves to either deliver ascorbic acid to tissues with high demand of vitamin C for enzymatic reactions or to protect metabolically highly active cells or protect specialized tissues from oxidative stress3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize SVCT2 from rat, mouse, and human samples.

For research purposes only, not for human use
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