Anti-Synapsin III (SYN3) Antibody

Synapsin-3
  • New
Cat #: ANR-017
Alternative Name Synapsin-3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RPPVQGRSTSQQGE, corresponding to amino acid residues 510-523 of rat Synapsin III (Accession O70441). Extracellular, C-terminus.
    Accession (Uniprot) Number O70441
    Gene ID 29130
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
      • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
        1,2. Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:400).
        3,4. Anti-Synapsin III (SYN3) Antibody, preincubated with the control peptide antigen.
        Western blot analysis human SH-SY5Y neuroblastoma cell line lysate:
        1. Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:200).
        2. Anti-Synapsin III (SYN3) Antibody, preincubated with the control peptide antigen.
      • Expression of Synapsin 3 in mouse and rat hippocampus
        Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SYN3 staining (green) in mouse hippocampal CA3 region is detected in the mossy fiber terminal (MFT) region. B. In rat hippocampal dentate gyrus (DG) region, SYN3 immunoreactivity (green) is observed in hilus of the dentate gyrus. Cell nuclei are stained with DAPI (blue).
      • Cruceanu, C. et al. (2013) Int. J. Neuropsychopharmacol. 16, 289.
      • Murphy, B.C. et al. (2008) BMC Med. Genet. 9, 115.
      • Cheng. Q. et al. (2018) Front. Synaptic Neurosci. 10, 33.
      • Gerth, F. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 12057.
      • Synapsin 3 (Syn3) belongs to the synapsin family of neuronal phosphoproteins, vesicle-associated proteins that includes two additional members: Synapsin I and Synapsin II. Alternative splicing of the genes results in at least ten variants with various levels of homology.

        Synapsins are involved in synaptogenesis, synaptic transmission and synaptic plasticity and are required for the formation and maintenance of the reserve and recycling pools of synaptic vesicles through association with the actin cytoskeleton. Several protein kinases, including PKA, calcium-calmodulin regulated protein kinases and the mitogen-activated protein kinase (MAPK) use synapsins as their substrates1-4.

        Syn3 plays an important role in several disorders, including bipolar disorder, schizophrenia and epilepsy1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 23/05/2019

    Anti-Synapsin III (SYN3) Antibody (#ANR-017) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin III from human, rat, and mouse samples.

    For research purposes only, not for human use