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Anti-Tetraspanin 7 (extracellular) Antibody

TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.

Cat #: ANR-187
Alternative Name TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RTYTDAMQTYNGNDER, corresponding to amino acid residues 122 - 137 of human Tetraspanin 7 (Accession P41732). Extracellular, 2nd loop.
Accession (Uniprot) Number P41732
Gene ID TSPAN7
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 15 out of 16 amino acid residues identical; rat – 14 out of 16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:500). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 4):
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 4):
    1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:400). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
Immunohistochemistry
  • Expression of Tetraspanin 7 in the rat substantia nigra region.
    Expression of Tetraspanin 7 in the rat substantia nigra region.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Tetraspanin 7 immunoreactivity (green) appears in neuronal profiles (arrows) in both pars reticulata (SNR) and pars compacta (SNC). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue).
  • Expression of Tetraspanin 7 in the mouse hippocampus.
    Expression of Tetraspanin 7 in the mouse hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region shows Tetraspanin 7 immunoreactivity (green) in neuronal profiles in the pyramidal (P) and stratum radiatum (SR, horizontal arrows) layers. Glial profiles appeared mainly in the stratum radiatum layer (vertical arrows). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Indirect flow cytometry
  • Cell surface detection of Tetraspanin 7 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Tetraspanin 7 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
     ___  Cells.
     ___  Cells + goat-anti-rabbit-FITC.
     ___  Cells + Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (2.5µg) + goat-anti-rabbit-FITC.
References
  1. Termini, C.M. et al. (2017) Front Cell Dev Biol, 5, 34
  2. Brieuc, P.P. et al. (2020) Med. Microbiol. Immunol. 209, 427.
  3. McLaughlin, K.A. et al. (2020) Med. Microbiol. Immunol. 209, 437.
Scientific background

Tetraspanins (Tspans) are a family of four-span transmembrane proteins, known as plasma membrane “master organizers.” They form Tspan-enriched microdomains (TEMs or TERMs) through lateral association with one another and other membrane proteins.1

Tetraspanins are characterized by four transmembrane domains, two extracellular loops: one small extracellular loop (SEL or EC1) and one larger extracellular loop (LEL or EC2), a short intracellular loop, and two short intracellular tails.1

Teatraspanin 7 (TSPAN7) is highly expressed in the brain and was first described as a candidate gene for X‐linked intellectual disability, probably through the modulation of surface expression of AMPA and dopamine receptors.2

Tetraspanin 7 also has a key role in controlling pancreatic β-cell Ca2+ handling and defining the set-point of insulin secretion through modulation of L-type Cav1.2 and Cav1.3 channels.  Furthermore, it has been identified as a target for autoantibodies in Type I diabetes. 3

In addition, Tetraspanin 7 is involved in the regulation of HIV virus entry into dendritic cells and several types of cancer progression. 2

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 24/01/2021

Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187) is a highly specific antibody directed against an extracellular epitope of human Tetraspanin 7. The antibody can be used in western blot, indirect flow cytometry and immunohistochemistry. It has been designed to recognize Tetraspanin 7 from mouse, rat and human samples.

For research purposes only, not for human use
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